Inhibition of glutathione synthesis decreases chilling tolerance in Chorispora bungeana callus

文献类型： 外文期刊

第一作者： Wu, Jianmin

作者： Wu, Jianmin;Zhao, Zhiguang;An, Lizhe;Liu, Yanhong;Xu, Shijian;Gao, Dahai;An, Lizhe;Zhang, Youfu;Liu, Yanhong

作者机构：

关键词： chilling tolerance;Chorispora bungeana;fatty acid;glutathione;plasma membrane

期刊名称：CRYOBIOLOGY （ 影响因子：2.487； 五年影响因子：2.641 ）

ISSN： 0011-2240

年卷期： 2008 年 57 卷 1 期

页码：

收录情况： SCI

摘要： The possible roles of reduced glutathione(GSH) in chilling tolerance were studied in callus generated from a representative alpine plant, Chorispora bungeana Fisch. & C.A. Mey (C. bungeana). The callus grew well under low-temperature and chilling treatment led only to slight injury, as indicated by a low level of ion leakage (IL). Malondialdehyde measurements also were not elevated, however GSH was. Exogencrusly application of L-buthionine-(S R)-sulfoximine (BSO), an inhibitor of gamma-glutamylcysteine synthetase (gamma-ECS), arrested the GSH accumulation induced by chilling and resulted in a significant decrease in cell growth and an increase in IL and malondialdehyde. These results implied that C. bungeana is a plant with a strong low-temperature tolerance mechanism, and the tolerance of C, bungeana may be associated with GSH accumulation. Under chilling treatment, the proportion of unsaturated fatty acid in the plasma membrane (PM) increased significantly in callus of C. bungeana mainly due to increases in C18:2 and C18:3, the membrane fluidity (indicated by DPH fluorescent polarization) however was maintained. High PM H+-ATPase activities were also induced by chilling. Exogenously application of BSO blocked the effects of chilling treatments on the changes of fatty acids and PM H+-ATPase activities, reducing the PM membrane fluidity. On the other hand, simultaneous application of GSH and BSO to callus under chilling treatments reversed the effects of BSO on the changes of fatty acids, PM fluidity and PM H+-ATPase activities. These results Suggested that GSH induced by low-temperature treatments may confer chilling tolerance to C. bungeana, probably by increasing unsaturated fatty acid compositions and maintaining PM fluidity and high enzymatic activity. (C) 2008 Elsevier Inc. All rights reserved.

分类号：

  • 相关文献

[1]Antifungal activity of eugenol against Botrytis cinerea. Wang, Chunmei,Chen, Hao,Fan, Yongjian,Shi, Zhiqi,Wang, Chunmei,Chen, Hao,Fan, Yongjian,Shi, Zhiqi,Wang, Chunmei,Chen, Hao,Fan, Yongjian,Shi, Zhiqi,Zhang, Jie. 2010

[2]Effects of salinity on activities of H+ -ATPase, H+-PPase and membrane lipid composition in plasma membrane and tonoplast vesicles isolated from soybean (Glycine max L.) seedlings. Yu, BJ,Lam, HM,Shao, GH,Liu, YL. 2005

[3]Plasma membrane NADPH oxidase in tobacco-tobacco mosaic virus interaction. Zhang, HM,Chen, J. 1999

[4]Inhibitory effect of chitosan on growth of the fungal phytopathogen, Sclerotinia sclerotiorum, and sclerotinia rot of carrot. Wang Qing,Zuo Jin-hua,Wang Qian,Na Yang,Gao Li-pu. 2015

[5]Relationship Between Seed Quality and Changes in Conjugated Polyamine in Plasma Membrane Purified from Wheat Embryos during Grain Ripening. Du, H. Y.,Liu, H. P.,Kurtenbach, R.,Du, H. Y.,Liu, G. T.,Zhou, X. G..

[6]Characterization and expression profiling of cucumber kinesin genes during early fruit development: revealing the roles of kinesins in exponential cell production and enlargement in cucumber fruit. Yang, Xue Yong,Wang, Yan,Jiang, Wei Jie,Liu, Xiao Ling,Zhang, Xiao Meng,Yu, Hong Jun,Huang, San Wen,Liu, Guo Qin. 2013

[7]Development of highly regenerable callus lines and Agrobacterium-mediated transformation of Chinese lawngrass (Zoysia sinica Hance) with a cold inducible transcription factor, CBF1. Li, RF,Wei, JH,Wang, HZ,He, J,Sun, ZY. 2006

[8]Genotype x environment interactions for chilling tolerance of rice recombinant inbred lines under different low temperature environments. Jiang, Wenzhu,Lee, Joohyun,Chu, Sang-Ho,Ham, Tae-Ho,Woo, Mi-Ok,Cho, Young-Il,Koh, Hee-Jong,Jiang, Wenzhu,Lee, Joohyun,Chu, Sang-Ho,Ham, Tae-Ho,Woo, Mi-Ok,Cho, Young-Il,Koh, Hee-Jong,Chin, Joong-Hyoun,Han, Longzhi,Xuan, Yingshi,Yuan, Donglin,Xu, Furong,Dai, Luyuan,Yea, Jong-Doo.

[9]Salicylic-Acid-Induced Chilling- and Oxidative-Stress Tolerance in Relation to Gibberellin Homeostasis, C-Repeat/Dehydration-Responsive Element Binding Factor Pathway, and Antioxidant Enzyme Systems in Cold-Stored Tomato Fruit. Ding, Yang,Zhao, Jinhong,Nie, Ying,Fan, Bei,Wu, Shujuan,Zhang, Yu,Tang, Xuanming,Sheng, Jiping,Shen, Lin,Zhao, Ruirui.

[10]Low-temperature conditioning induces chilling tolerance in 'Hayward' kiwifruit by enhancing antioxidant enzyme activity and regulating en-dogenous hormones levels. Yang, Qingzhen,Rao, Jingping,Wang, Yuping,Sun, Zhenying,Ma, Qiushi,Dong, Xiaoqing,Yang, Qingzhen,Zhang, Zhengke. 2013

[11]Stress-responsive gene ICE1 from Vitis amurensis increases cold tolerance in tobacco. Dong, Chang,Zhang, Zhen,Ren, Junpeng,Huang, Jinfeng,Wang, Yan,Cai, Binhua,Tao, Jianmin,Dong, Chang,Qin, Yang,Wang, Bailin. 2013

[12]Effects of Postharvest Gibberellic Acid Treatment on Chilling Tolerance in Cold-Stored Tomato (Solanum lycopersicum L.) Fruit. Zhu, Zhen,Ding, Yang,Zhao, Jinhong,Nie, Ying,Zhang, Yu,Tang, Xuanming,Sheng, Jiping.

[13]Variation of photosynthetic tolerance of rice cultivars (Oryza sativa L.) to chilling temperature in the light. Li, Xia,Cao, Kun,Wang, Chao,Sun, Zhi-wei,Yan, Lina. 2010

[14]A P4-ATPase Gene GbPATP of Cotton Confers Chilling Tolerance in Plants. Liu, Tingli,Guo, Shiwei,Lian, Ziyi,Chen, Fei,Yang, Yuwen,Chen, Tianzi,Ling, Xitie,Liu, Aiming,Zhang, Baolong,Wang, Rongfu.

[15]Effects of Calcium and Calmodulin Antagonists on Chilling Stress-Induced Proline Accumulation in Jatropha curcas L.. Yang, Shuang-Long,Deng, Feng-Fei,Gong, Ming,Lan, Shan-Shan.

[16]Molecular characterization of a cold-responsive RING-H2 finger gene from banana fruit and its interaction with MaMYC2a. Chen, Jiao,Kuang, Jian-Fei,Shan, Wei,Wang, Jun-ning,Xiao, Yun-yi,Chen, Jian-ye,Lu, Wang-jin,Chen, Jiao,Wang, Jun-ning.

[17]Cucumber (Cucumis sativus L.) over-expressing cold-induced transcriptome regulator ICE1 exhibits changed morphological characters and enhances chilling tolerance. Liu, Liying,Duan, Liusheng,Zhang, Jiachang,Zhang, Zhenxian,Ren, Huazhong,Mi, Guoquan.

[18]Responses of the hepatic glutathione antioxidant defense system and related gene expression in juvenile common carp after chronic treatment with tributyltin. Li, Zhi-Hua,Li, Ping,Shi, Ze-Chao,Li, Zhi-Hua,Li, Ping. 2015

[19]Modulation of glutathione-related antioxidant defense system of fish chronically treated by the fungicide propiconazole. Li, Zhi-Hua,Zlabek, Vladimir,Grabic, Roman,Li, Ping,Randak, Tomas,Li, Zhi-Hua,Li, Ping,Grabic, Roman. 2010

[20]Exogenous glutathione supplementation in culture medium improves the bovine embryo development after in vitro fertilization. Sun, Wei-Jun,Pang, Yun-Wei,Liu, Yan,Hao, Hai-Sheng,Zhao, Xue-Ming,Qin, Tong,Zhu, Hua-Bin,Du, Wei-Hua.

作者其他论文 更多>>

微信小程序