Molecular characterization, expression and association analysis of the porcine CMYA4 gene with carcass traits

文献类型： 外文期刊

第一作者： Xu, X. L.

作者： Xu, X. L.;Li, K.;Peng, Z. Z.;Zhao, S. H.;Yu, M.;Fan, B.;Zhu, M. J.;Liu, B.;Xu, X. L.;Li, K.;Peng, Z. Z.;Zhao, S. H.;Yu, M.;Fan, B.;Zhu, M. J.;Liu, B.;Li, K.;Xu, S. P.;Du, Y. Q.

作者机构：

关键词： association analysis;CMYA4;PCR-restriction fragment length polymorphism;porcine;single nucleotide polymorphism (SNP)

期刊名称：JOURNAL OF ANIMAL BREEDING AND GENETICS （ 影响因子：2.38； 五年影响因子：2.54 ）

ISSN： 0931-2668

年卷期： 2008 年 125 卷 4 期

页码：

收录情况： SCI

摘要： CMYA4 (cardiomyopathy-associated 4) gene plays an important role in thick filament assembly. In this study, we obtained the mRNA sequence including the full coding sequence and the partial 5' untranslated region of the porcine CMYA4 gene by using the rapid amplification of cDNA ends and reverse transcriptase polymerase chain reaction (RT-PCR) and the sequence was deposited in the GenBank nucleotide database (DQ_286571). The human (NM_173167) and mouse (NM_178680) homologues have a 91% and 87% identity with the porcine CMYA4 gene, respectively. The sequence contains an open reading frame encoding 930 amino acid residues, and the amino terminus of the predicted CMYA4 protein contains three tandem repeats belonging to the tetratricopeptide repeat family. Semi-quantitative RT-PCR results showed that the porcine CMYA4 gene is expressed exclusively in striated muscle tissue. An A558G single nucleotide polymorphism in the CMYA4 intron 15 detected as an MspI PCR- restriction fragment length polymorphism showed allele frequency differences among 225 unrelated pigs from six breeds. Association of the genotypes with growth and carcass traits showed that different genotypes of the CMYA4 gene were significantly associated with the backfat thickness of the area between sixth and seventh ribs (p < 0.05) and backfat thickness at the shoulder (p < 0.05).

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