A nonribosomal peptide synthetase gene tzw1 is involved in zwittermicin a biosynthesis in Bacillus thuringiensis G03

文献类型： 外文期刊

第一作者： Shao, Tiemei

作者： Shao, Tiemei;Zhang, Jie;Wang, Guangjun;Li, Zhuofu;Liu, Jianmi;Song, Fuping;Bai, Linquan;Bai, Linquan;Liu, Daqun

作者机构：

期刊名称：CURRENT MICROBIOLOGY （ 影响因子：2.188； 五年影响因子：2.197 ）

ISSN： 0343-8651

年卷期： 2008 年 57 卷 1 期

页码：

收录情况： SCI

摘要： A 4.20-kb SspI fragment from Bacillus thuringiensis G03 was cloned and sequenced. Sequencing analysis revealed two complete open reading frames (ORF; tzw1 and tzw2), and one incomplete ORF (tzw3) (GenBank accession no. EU293887). Tzw1 encodes a putative nonribosomal peptide synthetase with thiolation and condensation domains localized at the C-termini, whereas tzw2 and tzw3 encode acyl carrier protein and Acyl-CoA dehydrogenase, respectively. To investigate the function of tzw1 in zwittermicin A (ZA) biosynthesis, an in-frame deletion of 1,461 bp within tzw1 was constructed. The mutant abolished ZA production. Complementation of the mutant with cloned tzw1 restored ZA productivity. These results revealed that tzw1 is required for ZA biosynthesis in B. thuringiensis G03.

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