Identification of microsatellites in cattle unigenes

文献类型： 外文期刊

第一作者： Yan, Qiuliang

作者： Yan, Qiuliang;Lib, Hongbin;Wei, Caihong;Niu, Lili;Li, Shangang;Du, Lixin;Yan, Qiuliang;Zhang, Yinghan;Guan, Shan

作者机构：

关键词： cattle;microsatellite;expressed sequence tags;EST-SSR

期刊名称：JOURNAL OF GENETICS AND GENOMICS （ 影响因子：4.275； 五年影响因子：5.223 ）

ISSN： 1673-8527

年卷期： 2008 年 35 卷 5 期

页码：

收录情况： SCI

摘要： To identify EST-SSR molecular markers, 41,986 cattle UniGene sequences from NCBI were mined for analyzing SSRs. A total of 1,831 SSRs were identified from 1,666 ESTs, which represented an average density of 19.88 kb per SSR. The frequency of EST-SSRs was 4.0%. The dinucleotide repeat motif was the most abundant SSR, accounting for 54%, followed by 22%, 13%, 7% and 4%, respectively, for tri-, hexa-, penta- and tetra-nucleotide repeats. Depending upon the length of the repeat unit, the length of microsatellites varied from 14 to 86 bp. Among the di- and tri-nucleotide repeats, AC/TG (57%) and AGC (12%) were the most abundant type. Annotation of EST-SSRs was also carried out. Three hundred primer pairs were randomly designed using Prime Premier 5.0 program and Oligo 5.0 for further experimental validation.

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