Rapid detection of porcine circovirus type 2 by loop-mediated isothermal amplification

文献类型: 外文期刊

第一作者: Chen, Hao-tai

作者: Chen, Hao-tai;Zhang, Jie;Sun, De-hui;Chu, Yue-feng;Cai, Xue-peng;Liu, Xiang-tao;Luo, Xue-nong;Liu, Yong-sheng;Liu, Qing

作者机构:

关键词: porcine circovirus type 2 (PCV2);detection;loop-mediated isothermal amplification (LAMP);sensitivity;specificity

期刊名称:JOURNAL OF VIROLOGICAL METHODS ( 影响因子:2.014; 五年影响因子:2.001 )

ISSN: 0166-0934

年卷期: 2008 年 149 卷 2 期

页码:

收录情况: SCI

摘要: A method of loop-mediated isothermal amplification (LAMP) was employed to develop a rapid and simple detection system for porcine circovirus type 2 (PCV2). The amplification could be finished in 60 min under isothermal condition at 64 degrees C by employing a set of four primers targeting the cap gene of PCV2. The LAMP assay showed higher sensitivity than the conventional PCR, with a detection limit of five copies per tube of purified PCV2 genomic DNA. No cross-reactivity was observed from the samples of other related viruses including porcine circovirus type 1 (PCV1), porcine parvovirus (PPV), porcine pseudorabies virus (PRV) and porcine reproductive and respiratory syndrome virus (PRRSV). The detection rate of PCV2 LAMP for 86 clinical samples was 96.5% and appeared greater than that of the PCR method. The LAMP assay reported can provide a rapid yet simple test of PCV2 suitable for laboratory diagnosis and pen-side detection due to ease of operation and the requirement of only a regular water bath or heat block for the reaction. (c) 2008 Elsevier B.V. All rights reserved.

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