Real time TaqMan RT-PCR assay for the detection of Cucumber green mottle mosaic

文献类型： 外文期刊

第一作者： Chen Hongyun

作者： Chen Hongyun;Chen Qing;Lin Shiming;Chen Hongyun;Zhao Wenjun;Zhu Shuifang;Gu Qinsheng

作者机构：

关键词： cucumber green mottle mosaic virus;tobamovirus;real time RT-PCR;quarantine virus

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN： 0166-0934

年卷期： 2008 年 149 卷 2 期

页码：

收录情况： SCI

摘要： A real time reverse-transcription polymerase chain reaction (RT-PCR) was developed for efficient detection of Cucumber green mottle mosaic virus (CGMMV). The method was designed to use a duo-primer system with a TaqMan probe targeting the conserved sequence in 3' noncoding region (NCR) of CGMMV to detect isolates of this virus collected in China. The sensitivity of the real time RT-PCR assay was 0.13 pg of total RNA or 50 molecules of RNA transcripts. This level of sensitivity indicated that the one step real time RT-PCR developed in the present study could be used for routine testing assays. The real time RT-PCR method could assist in the implementation of quarantine measures for prevention and control of the disease caused by CGMMV. (c) 2008 Elsevier B.V. All rights reserved.

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