Progesterone and 17 beta-estradiol, but not follicle stimulating hormone, alter the sex ratio of murine embryos fertilized in vitro

文献类型： 外文期刊

第一作者： Zhang, L.

作者： Zhang, L.;Du, W.;Lin, X.;Zhang, L.;Zhang, A.;Chen, H.

作者机构：

关键词： sex ratio;IVF;blastocyst;diplex nested PCR;balb/c mouse

期刊名称：THERIOGENOLOGY （ 影响因子：2.74； 五年影响因子：2.93 ）

ISSN： 0093-691X

年卷期： 2008 年 69 卷 8 期

页码：

收录情况： SCI

摘要： The objective was to determine the effects of adding progesterone, 17 beta-estradiol (17 beta-E-2), and FSH during in vitro fertilization on development and sex ratio of murine embryos. Progesterone (33 - 330 pg/mL), 17 beta-estradiol (17 beta-E-2, 10 - 70 pg/mL), and FSH (0.01 - 0.05 IU/mL), were added to human tubal fluid (HTF); this medium (with or without hormones) was used to pre-incubate sperm (2 h) and to co-incubate sperm and oocytes (6 h). Thereafter, the ova were washed and incubated in mM16 medium and embryo sex was determined (by PCR) on Day 4 (insemination = Day 0). There was no effect (P > 0.05) of hormone treatments on rates of cleavage (6 h after cessation of co-incubation with sperm). The only significant effects of added hormones on development were a decrease in the rate of development to at least the morula stage in 165 pg/mL progesterone (0.46 +/- 0.03 vs. 0.54 +/- 0.05 in the control, mean +/- S.D.; P < 0.05) and a decrease in the blastocyst rate in 0.03 IU/mL FSH (0.34 +/- 0.00 vs. 0.42 +/- 0.04 in the control, P < 0.05). However, the ratio of male to female embryos was 1.61 and 2.90 following the addition of 99 pg/mL progesterone and 70 pg/mL 17 beta-E-2, respectively; both of these ratios were different (P < 0.01) than in the control group (1.20). In contrast, the addition of FSH to the medium had no significant effect on this ratio (range, 0.78 - 1.02). We concluded that the addition of progesterone and estradiol to the media during in vitro fertilization did not enhance embryonic development, but significantly increased the proportion of male murine embryos. (c) 2008 Elsevier Inc. All rights reserved.

分类号：

  • 相关文献

[1]Optimization of cryopreservation of buffalo (Bubalus bubalis) blastocysts produced by in vitro fertilization and somatic cell nuclear transfer. Yang, C. Y.,Pang, C. Y.,Yang, B. Z.,Li, R. C.,Liang, X. W.,Lu, Y. Q..

[2]The BALB/c mouse infection model for improving the Haemophilus parasuis serotyping scheme. Yu, Jiang,Zhang, Yuyu,Du, Yijun,Li, Jun,Huang, Baohua,Sun, Wenbo,Cong, Xiaoyan,Peng, Jun,Ren, Sufang,Gou, Lihui,Shi, Jianli,Chen, Zhi,Chen, Lei,Li, Jianda,Yang, Jie,Wu, Jiaqiang,Wang, Jinbao,Yu, Jiang,Zhang, Yuyu,Du, Yijun,Li, Jun,Huang, Baohua,Sun, Wenbo,Cong, Xiaoyan,Peng, Jun,Ren, Sufang,Gou, Lihui,Shi, Jianli,Chen, Zhi,Chen, Lei,Li, Jianda,Yang, Jie,Wu, Jiaqiang,Wang, Jinbao,Peng, Jun.

[3]Production of transgenic blastocyst by nuclear transfer from different types of somatic cells in cattle. Gong, GC,Dai, YP,Fan, BL,Zhu, HB,Wang, HP,Wang, LL,Fang, CG,Wan, R,Liu, Y,Li, R,Li, N.

[4]In vitro development of donated frozen-thawed human embryos in a prototype static microfluidic device: a randomized controlled trial. Kieslinger, Dorit C.,Vergouw, Carlijn G.,Kostelijk, Elisabeth H.,Lambalk, Cornelis B.,Hao, Zhenxia,Le Gac, Severine,Hao, Zhenxia,Le Gac, Severine,Hao, Zhenxia.

[5]Trichostatin A Treatment on Two Types of Donor Cells for Somatic Cell Nuclear Transfer. Yao, Ya-Xin,Li, He-Ping,Yao, Ya-Xin,Guan, Wei-Jun,Li, Xiang-Chen,Su, Xiao-Hua,Ma, Yue-Hui. 2012

[6]The Evaluation of Factors Affected on Juvenile in vitro Embryo Transfer in Xinjiang Fine Wool Lamb. Wu, Weiwei,Wang, Feng,Wu, Weiwei,Hanikezi,Tian, Kechuan,Xu, Xinming,Tian, Yuezhen. 2012

[7]Separation of motile sperm for in vitro fertilization from frozen-thawed bull semen using progesterone induction on a microchip. Li, Jingchun,Ning, Bolin,Luo, Yinghua,Guo, Li,Wei, Guosheng,Liu, Shengjun,Zhang, Ying,Zhang, Aizhong,Wu, Rui,Li, Yanbing,Cao, Xinyan.

[8]A Comparison of in vitro Development of Buffalo (Bubalus bubalis) Embryos Produced either by Somatic Cell Nuclear Transfer or in vitro Fertilization using Oocytes Obtained by Different Methods. Yang, Chunyan,Pang, Chunying,Zheng, Haiying,Huang, Fenxiang,Shang, Jianghua,Wang, Jian,Yang, Bingzhuang,Liang, Xianwei. 2013

[9]In vitro embryo production in buffalo (Bubalus bubalis) using sexed sperm and oocytes from ovum pick up. Lu, Y. Q.,Lu, S. S.,Zhang, M.,Lu, K. H.,Liang, X. W.,Chen, M. T.,Zhang, X. F.,Pang, C. Y.,Huang, F. X.. 2008

[10]Interspecific competition between Peristenus spretus and Peristenus relictus (Hymenoptera: Braconidae), larval parasitoids of Apolygus lucorum (Hemiptera: Miridae). Wu, Kongming,Luo, Shuping,Zhang, Feng,Wu, Kongming,Zhang, Feng. 2018

[11]Comparative responses in rare minnow exposed to 17 beta-estradiol during different life stages. Liao, T.,Guo, Q. L.,Xu, Y.,Liao, T.,Cheng, W.,Jin, S. W.. 2009

[12]Migration and population structure characteristics of the small yellow croaker Larimichthys polyactis in the southern Yellow Sea. Xiong Ying,Yang Jian,Xiong Ying,Zhong Xiaming,Tang Jianhua,Yang Jian. 2016

[13]Effect of temperature on the reproductive biology of Peristenus spretus (Hymenoptera: Braconidae), a biological control agent of the plant bug Apolygus lucorum (Hemiptera: Miridae). Wu, Kongming,Luo, Shuping,Zhang, Feng,Wu, Kongming,Zhang, Feng.

[14]Host instar suitability in two invasive whiteflies for the naturally occurring parasitoid Eretmocerus hayati in China. Yang, Nian-Wan,Wan, Fang-Hao,Zhang, Yi-Bo,Sun, Li-Ying.

[15]Mammalian steroid hormones can reduce abundance and affect the sex ratio in a soil nematode community. Hu, Cheng,Hermann, Galit,Pen-Mouratov, Stanislav,Steinberger, Yosef,Hu, Cheng,Shore, Laurence. 2011

[16]Effects of transgenic Bt maize pollen on longevity and fecundity of Trichogramma ostriniae in laboratory conditions. Wu, Yan,He, Kang-Lai,Bai, Shu-Xiong. 2007

[17]Effects of heat shock on survival and reproduction of two whitefly species, Trialeurodes vaporariorum and Bemisia tabaci biotype B. Wan, Fanghao,Xie, Ming,Liu, Tongxian,Cui, Xuhong,Wan, Fanghao,Xie, Ming,Liu, Tongxian. 2008

[18]Gillnet Selectivity on the Small Yellow Croaker Larimichthys polyactis in the Southern Yellow Sea. Xiong, Ying,Jian, Yang,Xiong, Ying,Zhong, Xiaming,Tang, Jianhua,Jian, Yang,Li, Lingzhi. 2017

[19]Artificial gynogenesis and assessment of homozygosity in meiotic gynogens of spotted halibut (Verasper variegatus). Ji, Xiang-Shan,Chen, Song-Lin,Yang, Jing-Feng,Ma, Hong-Yu,Ji, Xiang-Shan,Jiang, Yun-Liang.

[20]The effects of relatedness on offspring sex ratio in pollinating fig wasps. Hu, Hao-Yuan,Niu, Li-Ming,Huang, Da-Wei,Hu, Hao-Yuan,Ma, Guang-Chang,Niu, Li-Ming,Fu, Yue-Guan,Peng, Zheng-Qiang,Huang, Da-Wei.

作者其他论文 更多>>

微信小程序