Identification of the avian infectious bronchitis coronaviruses with mutations in gene 3

文献类型： 外文期刊

第一作者： Liu, Shengwang

作者： Liu, Shengwang;Zhang, Qingxia;Chen, Jianfei;Han, Zongxi;Shao, Yuhao;Kong, Xiangang;Tong, Guangzhi

作者机构：

关键词： infectious bronchitis coronavirus;gene order;mutation;replication;pathogenicity

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN： 0378-1119

年卷期： 2008 年 412 卷 1-2 期

页码：

收录情况： SCI

摘要： The sequence of a 6.0-kb fragment was compared in the 3'-encoding region of the genome in 27 infectious bronchitis virus (IBV) strains. All these strains have the same S-3-M-5-N gene order, as is the case for other IBVs. However, the sizes of the corresponding open reading frames (ORFs) of some genes varied among the virus strains. Phylogenctic analysis and sequence alignments demonstrated that recombination events had occurred in the origin and evolution of the strains CK/CH/LSD/03I and CK/CH/LLN/981 and the possible recombinant junction sites might be located at the 3c and M genes, respectively. The normal product of ORF 3a is 57 amino acids long, whereas a 43-bp deletion at the 3'-end of the CK/CH/LSD/03I 3a gene was detected, resulting in a frameshift event and C-terminally truncated protein with 47 amino acids. Comparison of the growth ability in embryos and replication and pathogenicity in chickens with lBV carrying the normal 3a gene indicated that this deleted sequence in the 3a gene of CK/CH/LSD/031 was not necessary for viral pathogenesis and replication either in vitro or in vivo. Occurrence of a mutation at the corresponding position of the CK/CH/LLN/981 start codon in the 3a gene led to the absence of ORF 3a in this virus, resulting in a novel genomic organization at the 3'-encoding regions: S-3b, 3c-M-5a, 5b-N. Comparison with other viruses carrying the normal 3a gene revealed that CK/CH/LLN/98I had replication and pathogenicity abilities in vivo similar to those of other IBVs; however, its growth ability in embryos was lower, although the relationship between the lower growth ability and the ORF 3a defect requires further confirmation. (c) 2008 Elsevier B.V. All rights reserved.

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