A novel phytase gene appA from Buttiauxella sp GC21 isolated from grass carp intestine

文献类型： 外文期刊

第一作者： Shi, Pengjun

作者： Shi, Pengjun;Huang, Huoqing;Wang, Yaru;Luo, Huiying;Wu, Bo;Meng, Kun;Yang, Peilong;Yao, Bin

作者机构：

关键词： Buttiauxella sp.;grass carp;phytase;phytate

期刊名称：AQUACULTURE （ 影响因子：4.242； 五年影响因子：4.723 ）

ISSN： 0044-8486

年卷期： 2008 年 275 卷 1-4 期

页码：

收录情况： SCI

摘要： A novel phytase gene, appA, was isolated by degenerate PCR and genomic library screening from Buttiauxella sp. GC21, a bacterium isolated from grass carp intestine. The full-length gene consists of an open reading frame of 1341 bp and encodes 446 amino acids, including 33 amino acid residues of a putative signal peptide. Containing the active site of typical histidine acid phosphatases, the amino acid sequence shows maximum identity (71%) to PhyA from Obesumbacterium proteus. The gene encoding the mature phytase was expressed in Escherichia coli and the purified recombinant APPA had a specific activity for sodium phytate of 1180 U mg(-1) of protein. The optimum temperature was 55 degrees C and the optimum pH was 4.5. The K-m value was 0.36 mM, with a V-max of 1.39 mmol min(-1) mg(-1). This is the first report to detect and isolate a phytase from Buttiauxella sp. (c) 2008 Elsevier B.V. All rights reserved.

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