Genotype-dependent enhancement of tomato transformation and regeneration by GRF4 and GIF1

文献类型： 外文期刊

第一作者： Tang, Yaping

作者： Tang, Yaping;Shen, Xinyan;Deng, Xuan;Song, Lei;Liu, Bo;Lu, Yongen;Ouyang, Bo;Tang, Yaping;Zhang, Guoru;Yang, Shengbao

作者机构：

关键词： Tomato; Growth-regulating factors (GRFs); SlGRF4-GIF1; RUBY; Transformation

期刊名称：PLANT GROWTH REGULATION （ 影响因子：3.9； 五年影响因子：3.8 ）

ISSN： 0167-6903

年卷期： 2025 年

页码：

收录情况： SCI

摘要： Agrobacterium-mediated genetic transformation of plants, including tomato, faces challenges related to genotype variability and regeneration rates. Research suggests that growth-regulating factors (GRFs) and their interacting factors (GIFs) are key to enhancing transformation and regeneration in various plant species. However, their specific effects on tomato remain underexplored. In this study, we identified and analyzed 11 SlGRF homologous genes in tomato and validated the interaction between SlGRF4 and SlGIF1. In addition, we demonstrated the effectiveness of the RUBY visual marker system in tomato transgenic research. Developmental regulators significantly influenced callus transformation and shoot regeneration rates across three tomato genotypes, with the SlGRF4-GIF1 combination providing the most consistent enhancement in both processes. These findings hold promise for tomato improvement via genome editing.

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