Modified BIGH3 with an RGDRGD motif promotes human corneal epithelial cell adhesion and migration In Vitro

文献类型： 外文期刊

第一作者： Ge, Hongyan

作者： Ge, Hongyan;Cao, Wenping;Leng, Fei;Chen, Weiye;Liu, Ping;Chen, Weiye

作者机构：

关键词： adhesion;BIGH3;human corneal epithelial cell (HCE);migration;RGDRGD

期刊名称：CURRENT EYE RESEARCH （ 影响因子：2.424； 五年影响因子：2.439 ）

ISSN： 0271-3683

年卷期： 2008 年 33 卷 3 期

页码：

收录情况： SCI

摘要： Purpose: BIGH3 protein plays an important role in mediating human corneal epithelial (HCE) cell adhesion and migration. The aim of this study was to investigate the effects of native and modified BIGH3 protein containing an Arg-Gly-Asp-Arg-Gly-Asp (RGDRGD) motif on the adhesion and migration of HCE cells. Methods: A modified human BIGH3 gene containing an RGDRGD motif was obtained by rapid site-directed mutagenesis. Recombinant human native and modified BIGH3 proteins were then obtained and purified. The effects of the native and the modified version on the adhesion and migration of HCE cells were examined in the presence or absence of anti-31 antibody or anti-BIGH3 antibody or RGD peptide in vitro. Results: Recombinant native and modified BIGH3 proteins were successfully obtained and significantly promoted the adhesion and migration of human HCE cells in vitro, and the construct with the RGDRGD motif was more effective. The enhanced adhesion and migration were blocked by anti-31antibody or anti-BIGH3 antibody or RGD peptide. Conclusion: BIGH3 promotes HCE cell adhesion and migration; modified RGDRGD-BIGH3 was more effective than native BIGH, and this is mediated by the Arg-Gly-Asp (RGD) motif and 31integrin in a RGD-dependent manner.

分类号：

  • 相关文献

[1]A C-terminal fragment BIGH3 protein with an RGDRGD motif inhibits corneal neovascularization in vitro and in vivo. Ge, Hongyan,Tian, Pei,Guan, Linan,Yin, Xiuli,Liu, Hanruo,Xiao, Nan,Luo, Xin,Sun, Yunduan,Qi, Donghua,Ni, Shuang,Liu, Ping,Xiong, Yongzhong. 2013

[2]Genome shuffling of Lactobacillus plantarum C88 improves adhesion. Zhao, Yujuan,Duan, Cuicui,Gao, Lei,Yu, Xue,Niu, Chunhua,Li, Shengyu. 2017

[3]Adhesion capability of first two domains at N terminus of NP_785232 protein and their interaction with a UV-absorbing component from human mucus. Du, L.,Huo, G.,Du, L.,Ju, X.,Liu, F.. 2010

[4]Assessment of the role of brine shrimp Artemia in white spot syndrome virus (WSSV) transmission. Zhang, Jia-Song,Dong, Shuang-Lin,Dong, Yun-Wei,Tian, Xiang-Li,Zhang, Jia-Song,Cao, Yi-Cheng,Li, Zuo-Jia,Yan, Dong-Chun.

[5]Protective Immune Response of Bacterially-Derived Recombinant FaeG in Piglets. Huang Yahong,Liang, Wanqi,Pan, Aihu,Zhou, Zhiai,Wang, Qiang,Huang, Cheng,Chen, Jianxiu,Zhang, Dabing.

[6]Vibrio parahaemolyticus enolase is an adhesion-related factor that binds plasminogen and functions as a protective antigen. Jiang, Wei,Li, Xintong,Yi, Li,Liu, Yongjie,Jiang, Wei,Han, Xiangan,Wang, Quan,Ding, Chan.

[7]Adhesion Behavior of WC Coating Deposited on Titanium Alloy by Electrospark Deposition. He, P.,Qian, Y. Y.,Chang, Z. L.,Wang, R. J..

[8]Role of the ehxA gene from Escherichia coli serotype O82 in hemolysis, biofilm formation, and in vivo virulence. Jiang, Chenggang,An, Tongqing,Wang, Shujie,Wang, Gang,Si, Wei,Tu, Yabin,Liu, Yonggang,Wu, Jianan,Liu, Siguo,Cai, Xuehui.

[9]Comparison of adhesive gut bacteria composition, immunity, and disease resistance in juvenile hybrid tilapia fed two different Lactobacillus strains. Liu, Wenshu,Gu, Zemao,Liu, Wenshu,Ren, Pengfei,He, Suxu,Xu, Li,Yang, Yaling,Zhou, Zhigang.

[10]Expression and immunological characteristics of the surface-localized pyruvate kinase in Mycoplasma gallisepticum. He, Suibin,Qi, Jingjing,Yu, Shengqing,Tan, Lei,Bao, Shijun,Qiu, Xvsheng,Wang, Xiaolan,Ding, Chan,He, Suibin,Yin, Yuncong,Fei, Rongmei,Ding, Chan.

[11]The functions of the variable lipoprotein family of Mycoplasma hyorhinis in adherence to host cells. Xiong, Qiyan,Wang, Jia,Ji, Yan,Ni, Bo,Zhang, Bixiong,Ma, Qinghong,Wei, Yanna,Feng, Zhixin,Liu, Maojun,Shao, Guoqing,Xiao, Shaobo.

[12]In Vitro Protective Efficacy of Clostridium butyricum Against Fish Pathogen Infections. Gao, Quanxin,Sun, Peng,Peng, Shiming,Yin, Fei,Shi, Zhaohong,Gao, Quanxin,Xiao, Yingping,Ma, Xiangming.

[13]Characterization of the role in adherence of Mycoplasma hyorhinis variable lipoproteins containing different repeat unit copy numbers. Xiong, Qiyan,Zhang, Bixiong,Wang, Jia,Ni, Bo,Ji, Yan,Wei, Yanna,Feng, Zhixin,Liu, Maojun,Shao, Guoqing,Zhang, Bixiong,Xiao, Shaobo,Shao, Guoqing.

[14]Antimony release from contaminated mine soils and its migration in four typical soils using lysimeter experiments. Zhao, Long,Hou, Hong,Shangguan, Yu-xian,Qin, Yusheng,Zhang, Naiming.

[15]Relationship of flight and reproduction in beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae), a migrant lacking the oogenesis-flight syndrome. Jiang, Xing Fu,Luo, Li Zhi,Sappington, Thomas W.. 2010

[16]Spring migration and summer dispersal of Loxostege sticticalis (Lepidoptera : Pyralidae) and other insects observed with radar in Northern China. Wu, KM,Cheng, DF,Guo, YY. 2004

[17]Up-regulation of miR-182-5p predicts poor prognosis in patients with lung cancer and associates with tumor cell growth and migration. Shi, Yuanyuan,Liu, Hao,Du, Xiang,Liu, Xuedong,Liu, Jing. 2017

[18]Variation of pre-ovipositional period in the brown planthopper, Nilaparvata lugens, collected in tropical, subtropical and temperate Asia. Wada, T.,Ito, K.,Takahashi, A.,Tang, J.. 2007

[19]Do second generation Asia corn borer (Lepidoptera: Crambidae) immigrate to corn fields from alternate habitats?. Chen, Ri-Zhao,Li, Lian-Bing,Li, Peng-Pei,Klein, Michael G.,Li, Qi-Yun,Sheng, Cheng-Fa. 2015

[20]Different genetic structures revealed resident populations of a specialist parasitoid wasp in contrast to its migratory host. Wei, Shu-Jun,Zhou, Yuan,Fan, Xu-Lei,Cao, Li-Jun,Zhou, Yuan,Xu, Zai-Fu,Hoffmann, Ary A.,Chen, Xue-Xin. 2017

作者其他论文 更多>>

微信小程序