A new isolation method of beta-D-glucans from spent yeast Saccharomyces cerevisiae

文献类型： 外文期刊

第一作者： Liu, Xiao-Yorig

作者： Liu, Xiao-Yorig;Wang, Qlang;Cui, Steve W.;Liu, Hong-Zhi

作者机构：

关键词： beta-D-glucans;induced autolysis;homogenization;Saccharomyces cerevisiae

期刊名称：FOOD HYDROCOLLOIDS （ 影响因子：9.147； 五年影响因子：9.169 ）

ISSN： 0268-005X

年卷期： 2008 年 22 卷 2 期

页码：

收录情况： SCI

摘要： beta-D-glucans, which are widely distributed in the cell walls of microorganisms, mushrooms and plants, have received much attention with respect to their biological functions. Traditional method for preparing beta-D-glucans from Saccharomyces cerevisiae cells was based on repeated extractions with acid or alkali solutions, which resulted in degradation of the beta-D-glucan chains. A new mild method to extract beta-D-glucans from S. cerevisiae cells was proposed in this paper, which was composed of induced autolysis, water and organic solvent treatment, homogenization and protease hydrolysis. Compared with traditional isolation methods, this new method had distinct advantages that it gave high yields and purity of beta-D-glucans and kept beta-D-glucans native conformation. Furthermore, this isolation procedure did not pollute the environment and could be easily scaled-up to an industrial process. The experimental data indicated that each extraction step had its significant effect on P-D-glucans preparation, especially, the induced autolysis and homogenization. After 24h of induced autolysis, 48% (w/w) cellular substances were released, whereas the cell wall nearly remained intact. Treated by hot water, the mechanical strength of the yeast cell wall decreased with the removal of the mannoproteins, which caused the yeast cell walls easy to be disrupted. As a result, the ratio of broken yeast cell slightly exceeded 95% after three passes in homogenization treatment in 70 MPa. Finally, beta-D-glucans were obtained at a yield of 91% of the original ratio in the yeast cell walls and with a purity of up to 93% (w/w). (C) 2006 Elsevier Ltd. All rights reserved.

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