Silencing of puroindoline a alters the kernel texture in transgenic bread wheat

文献类型： 外文期刊

第一作者： He, Zhonghu

作者： He, Zhonghu;Xia, Lanqin;Geng, Hongwei;Chen, Xinmin;He, Zhonghu;Lillemo, Morten;Morris, Craig F.

作者机构：

关键词： bread wheat;grain hardness;puroindolines;over-expression;transformation

期刊名称：JOURNAL OF CEREAL SCIENCE （ 影响因子：3.616； 五年影响因子：3.891 ）

ISSN： 0733-5210

年卷期： 2008 年 47 卷 2 期

页码：

收录情况： SCI

摘要： Grain hardness is an important end-use quality parameter of bread wheat, and one of the most important characters for quality improvement. The objective of this study was to further understand the function of puroindolines and the underlying mechanism in the formation of kernel texture. The highly efficient expression vector p UBPa harboring puroindoline a (Pina) was introduced into the bread wheat cultivar Zhongyou 9507-60 via biolistic transformation and transgenic plants were obtained. The integration of the foreign Pina gene was confirmed by PCR and genomic DNA Southern blot analysis. The levels of friabilin on the surface of water-washed starch granules varied among the transgenic lines. SDS-PAGE analysis of Triton X-114 extracted protein showed that the PINA protein was absent in three transgenic lines, indicating that the endogenous Pina gene most likely had been co-suppressed by the over-expression of the Pina transgene. SKCS kernel hardness and scanning electron microscopy analysis further confirmed the changes of kernel texture in these lines. (c) 2007 Elsevier Ltd. All rights reserved.

分类号：

  • 相关文献

[1]A new puroindoline b mutation present in Chinese winter wheat cultivar Jingdong 11. Chen, F,He, ZH,Xia, XC,Lillemo, M,Morris, C. 2005

[2]Puroindoline grain hardness alleles in CIMMYT bread wheat germplasm. Lillemo, Morten,Chen, Feng,Xia, nchun Xia,William, Manilal,Pena, Roberto J.,Trethowan, Richard,He, Zhonghu. 2006

[3]Molecular and biochemical characterization of puroindoline a and b alleles in Chinese landraces and historical cultivars. Chen, F,He, ZH,Xia, XC,Xia, LQ,Zhang, XY,Lillemo, M,Morris, CF. 2006

[4]Histological and Ultrastructural Observation Reveals Significant Cellular Differences between Agrobacterium Transformed Embryogenic and Non-embryogenic Calli of Cotton. Shang, Hai-Hong,Liu, Chuan-Liang,Zhang, Chao-Jun,Li, Feng-Lian,Hong, Wei-Dong,Li, Fu-Guang.

[5]Molecular and biochemical characterization of puroindoline A and B alleles in chinese improved cultivars and landraces. He, Z. H.,Chen, F.,Xia, X. C.,Zhang, X. Y.,He, Z. H.,Lillemo, M.,Morris, C. F.. 2007

[6]Effect of the puroindoline locus and environment on Chinese fresh noodle texture. Yang, EN,Zou, YC,Chen, DS,Sheppard, J,Martin, D,Huang, S,Mares, D,Sutherland, MW. 2006

[7]Identification of allelic variations of puroindoline genes controlling grain hardness in wheat using a modified denaturing PAGE. Chang, Cheng,Zhang, Haiping,Xu, Jie,Li, Weihua,Liu, Guangtian,You, Mingshan,Li, Baoyun. 2006

[8]Genetic Variation of High Molecular Weight Glutenin Subunits in Wheat Accessions in China. Guo, Xiaomin,Li, Hongqin,Xiang, Jishan,Xu, Xin,Liu, Weihua,Gao, Ainong,Yang, Xinming,Li, Xiuquan,Li, Lihui,Wang, Ruihui.

[9]Molecular Characterization of MYB28 Involved in Aliphatic Glucosinolate Biosynthesis in Chinese Kale (Brassica oleracea var. alboglabra Bailey). Yin, Ling,Cao, Bihao,Lei, Jianjun,Chen, Guoju,Chen, Hancai. 2017

[10]Investigating the mechanisms of glyphosate resistance in goosegrass (Eleusine indica) population from South China. Zhang Chun,Feng Li,He Ting-ting,Yang Cai-hong,Chen Guo-qi,Tian Xing-shan. 2015

[11]Evolutionary and functional study of the CDPK gene family in wheat (Triticum aestivum L.). Li, Ai-Li,Zhu, Yuan-Fang,Tan, Xiao-Mei,Wang, Xiang,Wei, Bo,Guo, Han-Zi,Zhang, Zeng-Lin,Chen, Xiao-Bo,Zhao, Guang-Yao,Kong, Xiu-Ying,Jia, Ji-Zeng,Mao, Long,Tan, Xiao-Mei.

[12]Molecular characterization and functional analysis of TRAF6 in orange-spotted grouper (Epinephelus coioides). Li, Yan-Wei,Li, Xia,Xiao, Xi-Xi,Li, An-Xing,Zhao, Fei,Luo, Xiao-Chun,Dan, Xue-Ming.

[13]Expression-based genotyping of the rice blast resistance genes in the elite maintainer line Yixiang1B. Hu, Xiao-Hong,Zhao, Zhi-Xue,Zeng, Rui,Li, Heng-Jing,Li, De-Qiang,Fan, Jing,Li, Yan,Huang, Fu,Wang, Wen-Ming,Wang, Li,Hu, Xiao-Hong,Lin, Gang,Zhao, De-Ming,Zhao, Zhi-Xue,Zeng, Rui,Li, Heng-Jing,Li, De-Qiang,Fan, Jing,Li, Yan,Huang, Fu,Wang, Wen-Ming,Wang, Li,Hu, Xiao-Hong,Lin, Gang,Zhao, De-Ming,Shi, Jun,Zhao, Zhi-Xue,Zeng, Rui,Li, Heng-Jing,Li, De-Qiang,Fan, Jing,Li, Yan,Huang, Fu,Wang, Wen-Ming,Wang, Li,Lin, Gang,Zhao, De-Ming,Shi, Jun,Fan, Jing,Li, Yan,Wang, Wen-Ming.

[14]Gene expression profiling reveals U1 snRNA regulates cancer gene expression. Cheng, Zhi,Sun, Yu,Niu, Xiaoran,Shang, Yingchun,Gao, Shan,Zhang, Tao,Cheng, Zhi,Sun, Yu,Niu, Xiaoran,Shang, Yingchun,Gao, Shan,Zhang, Tao,Sun, Yu,Gao, Shan,Ruan, Jishou,Cheng, Zhi,Chen, Ze,Cheng, Zhi,Chen, Ze. 2017

[15]AAV2-mediated follistatin overexpression induces ovine primary myoblasts proliferation. Nazari, Mahmood,Zhang, Li,Zhao, Fuping,Wei, Caihong,Du, Lixin,Salabi, Fatemeh. 2014

[16]Over-Expression of Arabidopsis EDT1 Gene Confers Drought Tolerance in Alfalfa (Medicago sativa L.). Zheng, Guangshun,Fan, Cunying,Di, Shaokang,Pang, Yongzhen,Zheng, Guangshun,Fan, Cunying,Di, Shaokang,Pang, Yongzhen,Zheng, Guangshun,Wang, Xuemin,Pang, Yongzhen,Zheng, Guangshun,Fan, Cunying,Xiang, Chengbin. 2017

[17]An acidic beta-mannanase from Penicillium sp C6: gene cloning and over-expression in Pichia pastoris. Yao, Bin,Cai, Hongying,Shi, Pengjun,Huang, Huoqing,Luo, Huiying,Bai, Yingguo,Yang, Peilong,Meng, Kun,Yao, Bin. 2011

[18]ECTOPIC EXPRESSION OF SUBUNIT A OF VACUOLAR H+-ATPASE FROM APPLE ENHANCES SALT TOLERANCE IN TOBACCO PLANTS. Dong, Q. L.,Liu, D. D.,Wang, Q. J.,Fang, M. J.,Hao, Y. J.,Yao, Y. X.,Dong, Q. L.,Liu, D. D..

[19]Cloning and expression of cytochrome P450 CYP6B7 in fenvalerate-resistant and susceptible Helicoverpa armigera (Hubner) from China. Tang, T.,Cheng, Y.,Shui, R.,Zhang, W.,Qiu, L.,Zhang, H.,Tang, T.. 2010

[20]Cloning, over-expression and characterization of an alkali-tolerant endo-beta-1,4-mannanase from Penicillium freii F63. Wang, Yawei,Shi, Pengjun,Luo, Huiying,Bai, Yingguo,Huang, Huoqing,Yang, Peilong,Yao, Bin,Wang, Yawei,Xiong, Hairong. 2012

作者其他论文 更多>>

微信小程序