Glutathione S-transferases from the larval gut of the silkworm Bombyx mori: cDNA cloning, gene structure, expression and distribution

文献类型： 外文期刊

第一作者： Gui, Zhong Zheng

作者： Gui, Zhong Zheng;Kim, Bo Yeon;Lee, Kwang Sik;Wei, Ya Dong;Sohn, Hung Dae;Jin, Byung Rae;Gui, Zhong Zheng;Wei, Ya Dong;Guo, Xijie

作者机构：

关键词： Baculovirus expression vector;Bombyx mori;cDNA cloning;enzyme;gene structure;glutathione S-transferase;silkworm

期刊名称：EUROPEAN JOURNAL OF ENTOMOLOGY （ 影响因子：1.225； 五年影响因子：1.548 ）

ISSN： 1210-5759

年卷期： 2008 年 105 卷 4 期

页码：

收录情况： SCI

摘要： Two glutathione S-transferase (GST) cDNAs, GSTD2 and GSTS2, were cloned from the silkworm Bombyx mori. The B. mori GSTD2 (BmGSTD2) gene spans 4371 bp and consists of four introns and five exons that encode 222 amino acid residues. The deduced amino acid sequence of BmGSTD2 showed 58% protein sequence identity to the Delta-class GST of Maduca sexta. The B. mori GSTS2 (BmGSTS2) gene spans 3470 bp and consists of three introns and four exons that encode 206 amino acid residues. The deduced amino acid sequence of BmGSTS2 revealed 67%, 63%, and 61% protein sequence identities to the Sigma-class GSTs from B. mori, Platynota idaeusalis, and M. sexta, respectively. The BmGSTD2 and BmGSTS2 cDNAs were expressed as 25 kDa and 23 kDa polypeptides, respectively, in baculovirus-infected insect Sf9 cells. Northern blot and Western blot analyses showed that BmGSTD2 and BmGSTS2 were specifically expressed in three gut regions, indicating that the gut is the prime site for BmGSTD2 and BmGSTS2 synthesis in B. mori larvae.

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