文献类型: 外文期刊
第一作者: Feng, Zheng
作者: Feng, Zheng;Tang, Zhong-Lin;Li, Kui;Liu, Bang;Yu, Mei;Zhao, Shu-Hong
作者机构:
关键词: pig;BTG2;BTG3;anti-proliferation;muscle development;gene expression;C2Cl2 cells
期刊名称:GENE ( 影响因子:3.688; 五年影响因子:3.329 )
ISSN: 0378-1119
年卷期: 2007 年 403 卷 1-2 期
页码:
收录情况: SCI
摘要: BTG2 and BTG3 are two members of the B-cell translocation gene family with anti-proliferative properties. BTG 1 gene in this gene family has been reported to play a key role in muscle growth. In this study, we identified and characterized the porcine BTG2 and BTG3 genes, mapped the two genes to porcine chromosomes, and analyzed their expression differences in the longissimus dorci muscle of 33 dpc (day postconception), 65 dpc and 90 dpc in the lean Landrace and fatty Chinese Tongcheng pig breeds. Expression changes in differentiated C2C12 cells were also investigated with myogenin as internal control. The results showed that the porcine BTG2 and BTG3 genes were mapped on SSC9q21-25 and SSC13q47, respectively. BTG2 gene expressed at high levels in skeletal muscle and heart in both Tongcheng and Landrace pigs whereas BTG3 gene expressed at lower levels in skeletal muscle and heart than in other tissues. Furthermore, BTG3 expressed at higher levels in skeletal muscle of Tonghceng compared with Landrace pig. The expression of BTG2 and BTG3 was significantly different in skeletal muscle among different developmental stages and between the two breeds. Expression analysis in murine myoblast cells showed that both genes were induced in differentiated C2C12 cells, suggesting a role of them in myogenic differentiation. Our study indicated that BTG2 and BTG3, especially BTG3 gene, may be important genes for skeletal muscle growth. (c) 2007 Elsevier B.V All rights reserved.
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