Real-time PCR quantification of Bemisia tabaci (Homoptera : Aleyrodidae) B-biotype remains in predator guts

文献类型： 外文期刊

第一作者： Zhang, Gui-Fen

作者： Zhang, Gui-Fen;Lue, Zhi-Chuang;Wan, Fang-Hao;Loevei, Gabor L.

作者机构：

关键词： Bemisia tabaci B-biotype;food web relationship;generalist predator;gut content analysis;quantitative detection;TaqMan real-time PCR

期刊名称：MOLECULAR ECOLOGY NOTES （ 影响因子：2.384； 五年影响因子：2.551 ）

ISSN： 1471-8278

年卷期： 2007 年 7 卷 6 期

页码：

收录情况： SCI

摘要： The cotton whitefly, Bemisia tabaci (Gennadius) B-biotype, is fed on by a wide variety of generalist predators, but there is little information on these predator-prey interactions, especially under field conditions. In this study, a real- time polymerase chain reaction (PCR) assay was developed to quantify B. tabaci B-biotype remains in predator gut. The B. tabaci B-biotype genomic DNA copy number was referred to the actual amount of BT1 isolate, the B. tabaci B-biotype specific DNA fragment. The numbers of BT1 isolate in one B. tabaci B-biotype egg, individual adult and a single red-eyed nymph were 2.56 x 10(3), 2.56 x 10(4), and 1.29 x 10(4) copies, respectively. When Propylaea japonica adults fed on one, two, four, eight or 16 red-eyed nymphs, the detected numbers of BT1 isolate ranged from 2.77 x 10(4) to 4.05 x 10(5) copies, forming a strong linear relationship (R-2 = 0.9899). Following the consumption of two red-eyed nymphs, prey DNA was detectable in 100% of P. japonica at t = 0, decreasing to 80.0% and 60.0% after 1-4 h and 8 h of digestion, respectively, with 3.36 x 10(4)-1.25 x 10(3) BT1 isolate copies. The predation by field-collected predators, 26 larvae of P. japonica, and of Harmonia axyridis each, Chrysopa spp. larvae (Chrysopa pallens and C. formosa, 18 individuals in total), and a single adult of Scymnus hoffmanni, 19 adults of Orius sauteri and nine adult spiders (Erigonnidium graminicolum and Neoscona doenitzi), on B. tabaci B-biotype were quantified. Of the 99 analysed predator individuals, 3.65 x 10(2)-4.60 x 10(5) copies of BT1 isolate, equivalent to 0.8-18.8 red-eyed nymphs were detected. These results suggest that TaqMan real- time PCR technology may provide a rapid and sensitive method for quantifying B. tabaci B-biotype remains in predator guts and will be invaluable in assessing the food web relationship between prey and arthropod predators.

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