Three digoxigenin-labeled cDNA probes for specific detection of the natural population of Barley yellow dwarf viruses in China by dot-blot hybridization

文献类型： 外文期刊

第一作者： Sun, Bo

作者： Sun, Bo;Wang, Xifeng;Zheng, Chuanlin;Zhou, Guanghe

作者机构：

关键词： Barley yellow dwarf viruses (BYDVs);primer;RT-PCR DIG-labeled cDNA probe;dot-blot hybridization assay

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN： 0166-0934

年卷期： 2007 年 145 卷 1 期

页码：

收录情况： SCI

摘要： Three digoxigenin-labeled cDNA probes complementary to the coat protein (CP) and read-through protein gene sequences of Barley yellow dwarf virus - one each for three species, namely BYDV-GAV, GPV, and PAV - were synthesized for developing a specific and sensitive dot-blot hybridization detection assay for total RNA extracts from field-infected wheat plants. The sensitivity limit for BYDV-GAV, GPV, and PAV probes corresponded to 25 mu g, 31.25 mu g, and 62.5 mu g tissue/spot, respectively. The frequencies for each of the three species determined that BYDV-GAV was the most prevalent in 269 wheat samples collected from 5 agro-ecological areas in China during 2004-2006. The high sensitivity and reliability of the molecular hybridization assay described introduce an important alternative to serological methods for detecting BYDV. This is especially important in less developed countries like China, where appropriate antibodies for BYDV are not available. (c) 2007 Elsevier B.V. All rights reserved.

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