Transformation of GbSGT1 gene into banana by an Agrobacterium-mediated approach
文献类型: 外文期刊
第一作者: Li Shengjun
作者: Li Shengjun;Wen Ruiming;Pei Xinwu;Chen Shikai;Li Weimin;Jia Shirong
作者机构:
关键词: transgenic banana;Agrobacterium-mediated;GbSGT1
期刊名称:PROGRESS IN NATURAL SCIENCE ( 影响因子:3.607; 五年影响因子:4.795 )
ISSN: 1002-0071
年卷期: 2007 年 17 卷 10 期
页码:
收录情况: SCI
摘要: SGT1 is a homologue of the yeast ubiquitin ligase-associated protein. It controls some protein degradation and activates defense pathway in plants. Cotton GbSGT1 gene ( Gossypium barbadense) has been isolated and characterized in previous work. In this study, the plant expression vector pBSGT1 with bar gene as a selection agent was constructed and transgenic banana was obtained via Agrobacterium-mediated transformation with the assistance of particle bombardment and screened with PCR and Basta spreading on banana plant leaves. Estimating of transgenic banana plants for resistance to Panama wilt is in progress.
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