SAGE tag based cDNA microarray analysis during larval to pupal development and isolation of novel cDNAs in Bombyx mori

文献类型： 外文期刊

第一作者： Zhang, Yong

作者： Zhang, Yong;Huang, Jianhua;Jia, Shihai;Liu, Wenbin;Li, Muwang;Wang, Sibao;Miao, Xuexia;Xiao, Huasheng;Huang, Yongping

作者机构：

关键词： bombyx mori;cDNA;gene expression;GLGI;SAGE;microarray;silkworm

期刊名称：GENOMICS （ 影响因子：5.736； 五年影响因子：4.939 ）

ISSN： 0888-7543

年卷期： 2007 年 90 卷 3 期

页码：

收录情况： SCI

摘要： Many genes act together during the complex process of insect larval and pupal development. 20-Hydroxyecdysone interacts with juvenile hormone to control insect growth and development and then activates several transcription factors, i.e., Broad, E74, and E75, and, subsequently, the late target genes. To investigate this phenomenon, we used serial analysis of gene expression (SAGE) tag-based cDNA microarray analysis to monitor the global gene expression profile during larval development and larva-pupa metamorphosis of the silkworm Bombyx mori. Of the 330 clones that were dotted to the chip, 267 were obtained by generating longer cDNA fragments from SAGE tags for gene identification, and the others were obtained from SAGE tag-matched genes or expressed sequence tags from public databases. According to the gene expression profile, the genes were classified into 12 clusters using a self-organizing map analysis. The results were partially confirmed using real-time reverse transcription-polyrnerase chain reaction. We obtained 22 full-length cDNAs using rapid amplification of 5' cDNA ends, of which eight genes were novel in the silkworm. Our results indicated that use of a cDNA microarray based on SAGE tags is effective for identifying and examining some low-expression genes associated with insect development. (c) 2007 Elsevier Inc. All rights reserved.

分类号：

  • 相关文献

[1]Glutathione S-transferases from the larval gut of the silkworm Bombyx mori: cDNA cloning, gene structure, expression and distribution. Gui, Zhong Zheng,Kim, Bo Yeon,Lee, Kwang Sik,Wei, Ya Dong,Sohn, Hung Dae,Jin, Byung Rae,Gui, Zhong Zheng,Wei, Ya Dong,Guo, Xijie. 2008

[2]Linkage and mapping analyses of the densonucleosis non-susceptible gene nsd-Z in the silkworm Bombyx mori using SSR markers. Li, MW,Guo, QH,Hou, CX,Miao, XX,Xu, AY,Guo, XJ,Huang, YP. 2006

[3]Loss of Posterior Silk Gland Transcription Specificity of Fibroin Light Chain Promoter due to Absence of 41 bp Sequence Containing Possible Inhibitor Binding Sites. Guo, TQ,Wang, JY,Wang, SP,Guo, XY,Huang, KW,Huang, JT,Lu, CD.

[4]Analyzing genetic relationships in Bombyx mori using intersimple sequence repeat amplification. Li, Muwang,Hou, Chengxiang,Miao, Xuexia,Xu, Anying,Huang, Yongping.

[5]Molecular and biochemical characterization of manganese-containing superoxide dismutase from the silkworm, Bombyx mori. Yamamoto, K,Zhang, PB,He, NJ,Wang, YQ,Aso, Y,Banno, Y,Fujii, H. 2005

[6]The complete mitochondrial genome of Bombyx mori strain Baiyun (Lepidoptera: Bombycidae). Zhang, Huixian,Zhang, Huixian,Li, Fengbo,Meng, Zhiqi,Zhu, Xinrong.

[7]Morphological abnormalities and lethality in silkworm (Bombyx mori) larvae treated with high concentrations of insect growth-blocking peptide. Zou, Feng Ming,Lee, Kwang Sik,Wan, Hu,Jin, Byung Rae,Gui, Zhong Zheng. 2014

[8]In vivo analysis of fibroin heavy chain signal peptide of silkworm Bombyx mori using recombinant baculovirus as vector. Wang, SP,Guo, TQ,Guo, XY,Huang, JT,Lu, CD.

[9]Microarray analysis of the gene expression profile in the midgut of silkworm infected with cytoplasmic polyhedrosis virus. Wu, Ping,Wang, Xiu,Jiang, Yun-Feng,Guo, Xi-Jie,Wu, Ping,Qin, Guang-xing,Liu, Ting,Li, Mu-Wang,Guo, Xi-Jie.

[10]Differentially expressed genes in white egg 2 mutant of silkworm, Bombyx mori, at early embryo development stages. Zhang, Guozheng,Lou, Chengfu,Zhang, Guozheng,Wei, Yadong,Xia, Dingguo,Zhang, Yeshun,Deng, Bo,Liu, Ziyu,Zhang, Jiekui,Zhang, Guozheng,Wei, Yadong,Xia, Dingguo,Zhang, Yeshun. 2011

[11]Immunoglobulin superfamily is conserved but evolved rapidly and is active in the silkworm, Bombyx mori. Xia, Q.,Huang, L.,Xu, P.,Fang, T.,Xia, Q.,Cheng, T.,Duan, J.,Xia, Q..

[12]Xuhuai Goat H-FABP Gene Clone, Subcellular Localization of Expression Products and the Preparation of Transgenic Mice. Yin, Yan-hui,Li, Bi-chun,Wei, Guang-hui,Zhu, Cai-ye,Li, Wei,Zhang, Ya-ni,Du, Li-xin,Cao, Wen-guang.

[13]BIRC7 gene in channel catfish (Ictalurus punctatus): Identification and expression analysis in response to Edwardsiella tarda, Streptococcus iniae and channel catfish Hemorrhage Reovirus. Li, Min,Wang, Qi-Long,Chen, Song-Lin,Sha, Zhen-Xia,Li, Min,Liu, Yang.

[14]Genome Array on Differentially Expressed Genes of Skin Tissue in Cashmere Goat at Early Anagen of Cashmere Growth Cycle Using DNA Microarray. Di Jiang,Xu Xin-ming,Ainiwaer, Lazate,Zhang Yan-hua,Tian Ke-chuan,Yu Li-juan,Wu Wei-wei,Tulafu, Hanikezi,Fu Xue-feng,Yasen, Marzeya. 2014

[15]Global analysis of gene expression in flower buds of Ms-cd1 Brassica oleracea conferring male sterility by using an Arabidopsis microarray. Bonnema, Guusje,Kang, Jungen,Zhang, Guoyu,Fang, Zhiyuan,Wang, Xiaowu.

[16]Identification of tapetum-specific genes by comparing global gene expression of four different male sterile lines in Brassica oleracea. Ma, Yuan,Zhu, Yingguo,Ma, Yuan,Wu, Jian,Wang, Xiaowu,Kang, Jungen.

[17]Transcriptome responses involved in artemisinin production in Artemisia annua L. under UV-B radiation. Pan, Wei Song,Tian, Hao,Wang, Jian Wen,Zheng, Li Ping,Tian, Hao,Li, Wan Yi.

[18]Biochemical and transcriptome analyses of a novel chlorophyll-deficient chlorina tea plant cultivar. Wang, Lu,Yue, Chuan,Cao, Hongli,Zhou, Yanhua,Zeng, Jianming,Yang, Yajun,Wang, Xinchao,Wang, Lu,Zeng, Jianming,Yang, Yajun,Wang, Xinchao,Wang, Lu,Yue, Chuan,Cao, Hongli,Zhou, Yanhua,Zeng, Jianming,Yang, Yajun,Wang, Xinchao. 2014

[19]Characterization of the genome expression trends in the heading-stage panicle of six rice lineages. Dzikiewicz, Katherine M.,Li, Lei,Peng, Zhi-Yu,Liu, Tingting,Deng, Xing-Wang,Peng, Zhi-Yu,Li, Songgang,Zhu, Qi-Hui,Zhang, Huiyong,Liu, Tingting,Ma, Ligeng,Deng, Xing-Wang,Zhang, Huiyong,Wang, Xiangfeng,Deng, Xing-Wang,Hu, Guocheng,Wei, Xinghua,Sun, Zongxiu,Zhu, Zhengge,Zhu, Qi-Hui,Ge, Song. 2009

[20]Identification of candidate genes involved in responses of Arabidopsis to polychlorinated biphenyls based on microarray analysis. Jin, Xiao-Fen,Shuai, Jian-Jun,Peng, Ri-He,Zhu, Bo,Fu, Xiao-Yan,Tian, Yong-Sheng,Zhao, Wei,Han, Hong-Juan,Chen, Chen,Xu, Jing,Yao, Quan-Hong,Xiong, Ai-Sheng,Qu, Shen-Chun.

作者其他论文 更多>>

微信小程序