Characterization, gene cloning, and expression of a novel xylanase XYNB from Streptomyces olivaceoviridis A1

文献类型： 外文期刊

第一作者： Wang, Yaru

作者： Wang, Yaru;Zhang, Honglian;He, Yongzhi;Luo, Huiying;Yao, Bin

作者机构：

关键词： Streptomyces olivaceoviridis A1;xylanase;XYNB;characterization;gene cloning;expression

期刊名称：AQUACULTURE （ 影响因子：4.242； 五年影响因子：4.723 ）

ISSN： 0044-8486

年卷期： 2007 年 267 卷 1-4 期

页码：

收录情况： SCI

摘要： A novel xylanase XYNB from Streptomyces olivaceoviridis Al was purified to electrophoretic homogeneity with the specific activity of 2869.78 U/mg, by ammonium sulfate precipitation, anion exchange and gel filtration chromatography. The purified XYNB was further characterized. The optimal pH and temperature for XYNB activity are 5.2 and 60 degrees C, respectively. Metal cations, EDTA and SDS had no or little effect on enzyme activity. The gene xynB coding mature protein of XYNB was cloned by degenerated PCR, which consisted of 576 bp and encoded 191 amino acid residues. The putative molecular weight of XYNB is about 20.8 kD. The xynB was expressed actively in both Escherichia coli and Pichia pastoris. In a 3 L fermentor, the expressed xylanase protein was accumulated up to 7 mg/mL in recombinant P. pastoris, and the xylanase activity exceeded 15,000 IU/mL. XYNB can be potentially utilized in fish feed industry. (C) 2007 Elsevier B.V. All rights reserved.

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