Karyotyping of Brassica oleracea L. based on cot-1 and ribosomal DNAs

文献类型： 外文期刊

第一作者： Wei, Wen-Hui

作者： Wei, Wen-Hui;Zhang, Su-Feng;Wang, Li-Jun;Chen, Bo;Wu, Xiao-Ming;Song, Yun-Chun

作者机构：

关键词： Brassica oleracea L;cot-1 DNA;karyotyping;ribosomal DNA

期刊名称：BOTANICAL STUDIES （ 影响因子：2.787； 五年影响因子：2.871 ）

ISSN： 1817-406X

年卷期： 2007 年 48 卷 3 期

页码：

收录情况： SCI

摘要： To explore a simple, reliable, and effective method of karyotyping Brassica oleracea L., Cot-1 DNA was isolated from its genome, labeled as probe with a Biotin-Nick Translation Mix kit, and in situ hybridized to mitotic spreads. Specific fluorescent bands appeared on each chromosome pair. 25S and 5S rDNAs were labeled as probes with a DIG-Nick Translation Mix kit and Biotin-Nick Translation Mix kit, respectively, and in situ hybridized to mitotic preparations. Signals could be detected on two chromosome pairs for 25S rDNA, and on only one for 5S rDNA. Cot-1 DNA contains rDNA. The site identity of Cot-1 DNA and 25S rDNA on the chromosome was determined by dual-colour fluorescence in situ hybridization (FISH). It showed that the karyotyping technique based on a combination of rDNA and Cot-1 DNA chromosome markers is a superior alternative. A more exact karyotype of B. oleracea has been developed based on rDNA locations and Cot-1 DNA fluorescent bands.

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