Molecular characterization of the soybean L-asparaginase gene induced by low temperature stress
文献类型: 外文期刊
第一作者: Cho, Chang-Woo
作者: Cho, Chang-Woo;Lee, Hye-Jeong;Chung, Eunsook;Kim, Kyoung Mi;Kim, Jee Eun Heo Jung-In;Chung, Jongil;Ma, Youzhi;Fukui, Kiichi;Lee, Dae-Won;Kim, Doh-Hoon;Chung, Young-Soo;Lee, Jai-Heou
作者机构:
关键词: fluorescence in situ hybridization;gene expression;low temperature;soybean;L-asparaginase;stress
期刊名称:MOLECULES AND CELLS ( 影响因子:5.034; 五年影响因子:4.932 )
ISSN: 1016-8478
年卷期: 2007 年 23 卷 3 期
页码:
收录情况: SCI
摘要: L-asparaginase (EC 3.5.1.1) catalyzes the hydrolysis of the amide group of L-asparagine, releasing aspartate and NH4+. We isolated a low temperature-inducible cDNA sequence encoding L-asparaginase from soybean leaves. The full-length L-asparaginase cDNA, designated GmASPI, contains an open reading frame of 1,258 bp coding for a protein of 326 amino acids. Genomic DNA blotting and fluorescence in situ hybridization showed that the soybean genome has two copies of GmASPI. GmASPI mRNA was induced by low temperature, ABA and NaCl, but not by heat shock or drought stress. E. coli cells expressing recombinant GmASPI had 3-fold increased L-asparaginase activity. A possible function of L-asparaginase in the early response to low temperature stress is discussed.
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