Isolation of cDNA sequences encoding the MAP kinase HOG1 and the MAP kinase kinase PBS2 genes of the fungus Alternaria tenuissma through a genetic approach

文献类型： 外文期刊

第一作者： Qiu, Dewen

作者： Qiu, Dewen;Jiang, Linghuo

作者机构：

关键词： Alternaria tenuissima;cDNA library;MAP kinase;HOG1;PBS2

期刊名称：JOURNAL OF MICROBIOLOGICAL METHODS （ 影响因子：2.363； 五年影响因子：2.24 ）

ISSN： 0167-7012

年卷期： 2007 年 69 卷 1 期

页码：

收录情况： SCI

摘要： Alternaria tenuissima is a fungus widely present in the environment and causes diseases in plants and humans in the world. In this study, we constructed an A. tenuissima cDNA expression library in a centromeric yeast vector that allows the isolation of functional cDNA sequences from this environmental and pathogenic fungus. Through a genetic approach we have isolated and functionally characterized the cDNA sequences encoding the MAP kinase (MAPK) Hog1p and the MAPK kinase Pbs2p of A. tenuissima. At HOG1 cDNA encodes a protein of 355 amino acids, while AtPBS2 cDNA encodes a protein of 683 amino acids. (c) 2007 Elsevier B.V. All rights reserved.

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