Characterization of M-class genome segments of muscovy duck reovirus S14

文献类型： 外文期刊

第一作者： Zhang, Yun

作者： Zhang, Yun;Guo, Dongchun;Geng, Hongwei;Liu, Ming;Hu, Qilin;Wang, Junwei;Tong, Guangzhi;Kong, Xiangang;Liu, Nihong;Liu, Chunguo

作者机构：

关键词： muscovy duck reovirus;M1, M2, and M3 genome segments;mu A, mu B, and mu NS protein;phylogenetic analysis;protein expression

期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）

ISSN： 0168-1702

年卷期： 2007 年 125 卷 1 期

页码：

收录情况： SCI

摘要： This report documents the first sequence analysis of the entire M1, M2, and M3 genome segments of the muscovy duck reovirus (DRV) S14. The complete sequence of each of the three M gene segments was determined. The M1 genome segment was 2283 nucleotides in length and was predicted to encode [LA protein of 732 residues. The Escherichia coli expressed M I transcripts generated a 108 kDa protein, as expected for mu A. A cleavage product of mu A, mu A1, could be detected by Western blotting with duck anti-reovirus and mouse anti-mu A polyclonal serum. mu A was distributed diffusely in the cytoplasma and nucleus of transfected Vero cells, which provides evidence that RA might be functional related to the mammalian reovirus (MRV) mu 2. The M2 gene was 2155 nucleotides in length and was predicted to encode mu B major outer capsid protein of 676 amino acids. The M3 genome segment was 1996 nucleotides in length and was predicted to encode a mu NS protein of 635 amino acids. It was unexpectedly found that 5 '-termini of the M1 and M2 genes ended with 5'-(A) under bar(U) under bar ACUUUU and 5'- U (U) under bar CUUU()under bar>UCUUUU, respectively, instead of 5'-GCUUUU, which is present on most mRNAs of other avian reoviruses (ARV). The UCAUC 3'-terminal sequences of the S14 M1, M2, and M3 genome segments are shared by DRV, ARV, and MRV. Alignment of the DRV mu A-, mu B-, and mu NS-encoding genes with ARV revealed 72.9-73.9%, 67.1-69.6%, and 69.4-70.8% nucleotide identity, respectively. The amino acid sequence homology between DRV and ARV ranged from 85.3 to 86.2% (mu A), 75.0 to 76.5% (mu B), and 78.4 to 79.8% (mu NS). Phylogenetic analyses of the M 1, M2, M3, and S-class [Kuntz-Simon, G., Le Gall-Recule, G., de Boisseson, C., Jestin, V., 2002. Muscovy duck reovirus crC protein is a typically encoded by the smallest genome segment. J. Gen. Virol. 83, 1189-1200; Zhang, Y., Liu, M., Hu, Q.L., Ouyang, S.D., Tong, G.Z., 2006a. Characterization of the (TC-encoding gene from muscovy duck reovirus. Virus Genes 36, 169-174; Zhang, Y., Liu, M., Ouyan, S.D., Hu, QL., Guo, D.C., Han, Z., 2006b. Detection and identification of avian, duck, and goose reoviruses by RT-PCR: goose and duck reoviruses aggregated the same specified genogroup in Orthoreovirus Genus II. Arch. Virol. 151, 1525-1538] genome segments suggests that DRV and ARV share a recent common ancestor and that the two lineages have subsequently undergone host dependent evolution. (c) 2006 Elsevier B.V. All rights reserved.

分类号：

  • 相关文献

[1]Characterization of the sigma B-encoding genes of muscovy duck reovirus: sigma C-sigma B-ELISA for antibodies against duck reovirus in ducks. Zhang, Yun,Guo, Dongchun,Liu, Ming,Geng, Hongwei,Hu, Qilin,Liu, Yin,Liu, Nihong. 2007

[2]Binding Characterization of Recombinant Odorant-binding Proteins from the Parasitic Wasp, Microplitis mediator (Hymenoptera: Braconidae). Zhang, Shuai.

[3]Molecular Cloning, and Characterization of an Adenylyl Cyclase-Associated Protein from Gossypium arboreum L.. Wang Sheng,Zhao Guo-hong,Jia Yin-hua,Du Xiong-ming. 2009

[4]Association of A-FABP gene polymorphism in intron 1 with meat quality traits in Junmu No. 1 white swine. Gao, Yan,Li, Fujuan,Wang, Shuai,Dai, Lisheng,Jiang, Hao,Liu, Dianfeng,Zhao, Zhihui,Zhang, Jiabao,Zhang, Yonghong,Sun, Boxing,Zhang, Shumin,Xiao, Shuqi. 2011

[5]Characterization of ribulose-1, 5-bisphosphate carboxylase/oxygenase and transcriptional analysis of its related genes in Saccharina japonica (Laminariales, Phaeophyta). Shao Zhanru,Li Qiuying,Yao Jianting,Duan Delin,Liu Fuli,Shao Zhanru,Li Qiuying. 2014

[6]Molecular cloning of a full-length cDNA for ECBP21 from Angelica dahurica. Mao, GH,Tang, WQ,Guo, Y,Ding, CB,Zhou, RG,Sun, DY. 2002

[7]Expression of organophosphorus hydrolase OPHC2 in Pichia pastoris: Purification and characterization. Chu, Xiao-Yu,Wu, Ning-Feng,Deng, Min-Jie,Tian, Jian,Yao, Bin,Fan, Yun-Liu.

[8]A novel self-cleavage system for production of soluble recombinant protein in Escherichia coli. Feng, Yufei,Wu, Donglai,Feng, Yufei,Xu, Qingyuan,Yang, Tao,Sun, Encheng,Li, Junping,Wu, Donglai,Shi, Dongfang.

[9]Cloning and expression analysis of a peptidoglycan recognition protein in silkworm related to virus infection. Gao, Kun,Qian, He-ying,Qin, Guang-xing,Hou, Cheng-xiang,Guo, Xi-jie,Gao, Kun,Qian, He-ying,Qin, Guang-xing,Hou, Cheng-xiang,Guo, Xi-jie,Gao, Kun,Deng, Xiang-yuan.

[10]Expression of proteins in superior and inferior spikelets of rice during grain filling under different irrigation regimes. Chen, Tingting,Xu, Genwen,Wang, Zhiqin,Zhang, Hao,Yang, Jianchang,Chen, Tingting,Zhang, Jianhua,Zhang, Jianhua.

[11]Improvement of ATP regeneration efficiency and operation stability in porcine interferon-alpha production by Pichia pastoris under lower induction temperature. Gao, Minjie,Wu, Jianrong,Zheng, Zhiyong,Shi, Zhongping,Zhan, Xiaobei,Dong, Shijuan,Yu, Ruisong. 2011

[12]Improvement of specific growth rate of Pichia pastoris for effective porcine interferon-alpha production with an on-line model-based glycerol feeding strategy. Gao, Min-Jie,Zheng, Zhi-Yong,Wu, Jian-Rong,Jin, Hu,Zhan, Xiao-Bei,Lin, Chi-Chung,Dong, Shi-Juan,Li, Zhen. 2012

[13]Regulation of cry Gene Expression in Bacillus thuringiensis. Peng, Qi,Song, Fuping,Deng, Chao,Lereclus, Didier,Lereclus, Didier. 2014

[14]Proteomic analysis of PEG-simulated drought stress-responsive proteins of rice leaves using a pyramiding rice line at the seedling stage. Xiong, Jian-Hua,Li, Yang-Sheng,Xiong, Jian-Hua,Fu, Bin-Ying,Xu, Hua-Xue.

[15]Thioredoxin reductase from Toxoplasma gondii: an essential virulence effector with antioxidant function. Xue, Junxin,Jiang, Wei,Chen, Yongjun,Wang, Minyan,Wang, Quan,Xue, Junxin,Gong, Fengju,Zeng, Peng,Xia, Can,Huang, Kehe,Xue, Junxin.

[16]Prokaryotic expression, identification and bioinformatics analysis of fbpB-esxA fusing gene from Mycobacterium tuberculosis. Wu, Qiang,Zhou, Peng,Wu, Qiang,Fu, Qiongyao,Cai, Qunfang,Fan, Zhigang,Zhan, Zhinong,Niu, Lina,Pei, Hua,Wu, Qiang,Zhou, Peng,Chen, Qian. 2011

[17]FUNCTIONAL CHARACTERIZATION AND IMMUNOLOCALIZATION OF ODORANT BINDING PROTEIN 1 IN THE LUCERNE PLANT BUG, Adelphocoris lineolatus (GOEZE). Wang, Gui-Rong,Zhang, Xue-Ying,Guo, Yu-Yuan,Zhang, Yong-Jun,Wang, Wei-Xuan,Zhang, Ziding,Zhou, Jing-Jiang. 2011

[18]Improving Performance and Operational Stability of Porcine Interferon-alpha Production by Pichia pastoris with Combinational Induction Strategy of Low Temperature and Methanol/Sorbitol Co-feeding. Gao, Min-Jie,Zhan, Xiao-Bei,Gao, Peng,Zhang, Xu,Shi, Zhong-Ping,Lin, Chi-Chung,Dong, Shi-Juan,Li, Zhen.

[19]A Time-Course Proteomic Analysis of Rice Triggered by Plant Activator BTH. Chen, Zhuo,Chen, Binghua,Guo, Qin,Chen, Zhuo,Chen, Binghua,Guo, Qin,Shi, Li,He, Ming,Qin, Zhiyang,Li, Lianhua,He, Peng,Wang, Zhenchao,Hu, Deyu,Yang, Song.

[20]High level soluble expression and one-step purification of IBDV VP2 protein in Escherichia coli. Jiang, Dawei,Zhang, Gaiping,Yang, Guoyu,Song, Yapeng,Wang, Guoqiang,Wang, Jucai,Zhao, Baolei,Jiang, Dawei,Liu, Yunchao,Zhang, Gaiping,Ji, Pengchao,Liu, Chang,Song, Yapeng,Su, Yunfang,Wang, Guoqiang,Wang, Jucai,Zhao, Baolei,Deng, Ruiguang,Wang, Aiping,Chen, Yumei.

作者其他论文 更多>>

微信小程序