NF-kappa B mediates the transcription of mouse calsarcin-I gene, but not calsarcin-2, in C2C12 cells

文献类型： 外文期刊

第一作者： Wang, Heng

作者： Wang, Heng;Yang, Shulin;Yang, E.;Zhu, Zhengmao;Mu, Yulian;Feng, Shutang;Li, Kui

作者机构：

期刊名称：BMC MOLECULAR BIOLOGY （ 影响因子：2.946； 五年影响因子：3.192 ）

ISSN： 1471-2199

年卷期： 2007 年 8 卷

页码：

收录情况： SCI

摘要： Background: The calsarcins comprise a novel family of muscle- specific calcineurin- interaction proteins that play an important role in modulating both the function and substrate specificity of calcineurin in muscle cells. The expression of calsarcin-1 (CS-1) is restricted to slow-twitch skeletal muscle fibres, whereas that of both calsarcin-2 (CS-2) and calsarcin-3 (CS-3) is enriched in fast-twitch fibres. However, the transcriptional control of this selective expression has not been previously elucidated. Results: Our real-time RT-PCR analyses suggest that the expression of CS-1 and CS-2 is increased during the myogenic differentiation of mouse C2C12 cells. Promoter deletion analysis further suggests that an NF-kappa B binding site within the CS-1 promoter is responsible for the up-regulation of CS- 1 transcription, but no similar mechanism was evident for CS-2. These findings are further supported by the results of EMSA analysis, as well as by overexpression and inhibition experiments in which NF-.B function was blocked by treatment with its inhibitor, PDTC. In addition, the overexpression of NFATc4 induces both the CS-1 and CS-2 promoters, whereas MEF2C only activates CS-1. Conclusion: Our present data suggest that NF-.B is required for the transcription of mouse CS-1 but not CS-2, and that the regulation of the calsarcins is mediated also by the NFAT and MEF2 transcription factors. These results provide new insights into the molecular mechanisms governing transcription in specific muscle fibre cells. The calsarcins may also serve as a valuable mechanistic tool to better understand the regulation of calcineurin signalling during muscle differentiation.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序