Assembly of pseudorabies virus genome-based transfer vehicle carrying major antigen sites of S gene of transmissible gastroenteritis virus: Potential perspective for developing live vector vaccines

文献类型： 外文期刊

第一作者： Yin, Jiechao

作者： Yin, Jiechao;Ren, Xiaofeng;Tian, Zhijun;Li, Yijing

作者机构：

关键词： pseudorabies virus;transmissible gastroenteritis;genome-based transfer;live vector vaccine

期刊名称：BIOLOGICALS （ 影响因子：1.856； 五年影响因子：2.05 ）

ISSN： 1045-1056

年卷期： 2007 年 35 卷 1 期

页码：

收录情况： SCI

摘要： Two severe porcine infectious diseases, pseudorabies (PR) and transmissible gastroenteritis (TGE) caused by pseudorabies virus (PRV) and transmissible gastroenteritis virus (TGEV) respectively often result in serious economic loss in animal husbandry worldwide. Vaccination is the important prevention means against both infections. To achieve a PRV genome-based virus live vector, aiming at further TGEV/PRV bivalent vaccine development, a recombinant plasmid pUG was constructed via inserting partial PK and full-length gG genes of PRV strain Bartha K-61 amplified into pUC119 vector. In parallel, another recombinant pHS was generated by introducing a fragment designated S 1 encoding the major antigen sites of S gene from TGEV strain TH-98 into a prokaryotic expression vector pP(RO)EX HTc. The SV40 polyA sequence was then inserted into the downstream of S1 fragment of pHS. The continuous region containing S1 fragment, SV40 polyA and four single restriction enzyme sites digested from pHS was subcloned into the downstream of gG promoter of pUG. In addition, a LacZ reporter gene was introduced into the universal transfer vector named pUGS-LacZ. Subsequently, a PRV genome-based virus live vector was generated via homologous recombination. The functionally effective vector was purified and partially characterized. Moreover, the potential advantages of this system are discussed. (c) 2006 The International Association for Biologicals. Published by Elsevier Ltd. All rights reserved.

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