A genome-wide microsatellite polymorphism database for the Indica and Japonica rice

文献类型: 外文期刊

第一作者: Zhang, Zhonghua

作者: Zhang, Zhonghua;Deng, Yajun;Tan, Jun;Hu, Songnian;Yu, Jun;Xue, Qingzhong

作者机构:

关键词: polymorphism;microsatellite or MS;database;whole-genome sequences;rice

期刊名称:DNA RESEARCH ( 影响因子:4.458; 五年影响因子:5.371 )

ISSN: 1340-2838

年卷期: 2007 年 14 卷 1 期

页码:

收录情况: SCI

摘要: Microsatellite (MS) polyinorphism is an important source of genetic diversity, providing support for map-based cloning and molecular breeding. We have developed a new database that contains 52 845 polyniorphic MS loci between indica and japonica, composed of ample Class II MS markers, and integrated IS 828 MS loci from IRGSP and genetic markers from RGP. Based on genetic marker positions on the rice genome (http://rise.genomics.org.cn/ rice2/index.jsp), we determined the approximate genetic distances of these MS loci and validated 100 randomly selected markers experimentally with 90% success rate. In addition, we recorded polymorphic MS positions in indica, cv. 9311 that is the most important paternal parent of the two-line hybrid rice in China. Our database will undoubtedly facilitate the application of MS markers in genetic researches and marker-assisted breeding. The data set is freely available from www.wigs.zju.edu.cn/achievrnerit/polySSR.

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