Long terminal repeats are not the sole determinants of virulence for equine infectious anemia virus

文献类型： 外文期刊

第一作者： Tu, Y. -B.

作者： Tu, Y. -B.;Zhou, T.;Yuan, X. -F.;Qiu, H. -J.;Xue, F.;Sun, C. -Q.;Wang, L.;Wu, D. -L.;Peng, J. -M.;Kong, X. -G.;Tong, G. -Z.

作者机构：

期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）

ISSN： 0304-8608

年卷期： 2007 年 152 卷 1 期

页码：

收录情况： SCI

摘要： The long terminal repeats (LTRs) of equine infectious anemia virus donkey leukocyte-attenuated virus (EIAV-DLA) were substituted with those of the wild-type EIAV-L (wt EIAV-L, the parent virus of EIAV-DLA). The resulting chimeric plasmid was designated pOK-LTR DLA/L. Purified pOK-LTR DLA/L was transfected into monocyte-derived macrophage (MDM) cultures prepared from EIAV-negative, heparinized whole blood from a donkey. Eighth-passage cell cultures developed the typical cytopathogenic effects (CPE) of EIAV infection, and virions with typical EIAV profiles were observed with an electron microscope. Horses were inoculated with the chimeric virus or EIAV-DLA and challenged with the wt EIAV-L strain six months later. All of the horses inoculated with either the chimeric virus or EIAV-DLA were protected from disease, whereas the control horses died with typical EIA symptoms.

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