Multiple transgenes Populus xeuramericana 'Guariento' plants obtained by biolistic bombardment

文献类型: 外文期刊

第一作者: Wang JianGe

作者: Wang JianGe;Su XiaoHua;Ji LiLi;Zhang BingYu;Hu ZanMin;Huang RongFeng;Tian YingChuan

作者机构:

关键词: Populus xeuramericana 'Guariento';biolistic bombardment;multi-gene;co-transformation;transgene

期刊名称:CHINESE SCIENCE BULLETIN ( 影响因子:1.649; 五年影响因子:1.738 )

ISSN: 1001-6538

年卷期: 2007 年 52 卷 2 期

页码:

收录情况: SCI

摘要: A JERF36 regulation gene, a selection marker gene (NPT-II), and the foreign genes levansucrase (SacB), Vitreoscilla hemoglobin (vgb), and Binary coleopterus insect resistance (BtCry3A+OC-I) were co-transferred into Populus xeuramericana 'Guariento' using biolistic bombardment; 25 kanamycin resistant plants were obtained. The results of PCR and Southern hybridization showed that the foreign genes had been integrated into the genome of P xeuramericana 'Guariento' and 5 genes were all transferred into 7 poplar plants. The results of a BtCry3A ELISA experiment indicated that the BtCry3A gene was expressed in the 7 transgenic poplar plants, and these plants grew well on coastal saline land.

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