Effect of beta-mercaptoethanol and buffalo follicular fluid on fertilization and subsequent embryonic development of water buffalo (Bubalus bubalis) oocytes derived from in vitro maturation

文献类型： 外文期刊

第一作者： Shang, J. H.

作者： Shang, J. H.;Huang, Y. J.;Zhang, X. F.;Huang, F. X.;Qin, J.

作者机构：

关键词： beta-mercaptoethanol (beta-ME);Follicular fluid;Oocyte;Water buffalo

期刊名称：ITALIAN JOURNAL OF ANIMAL SCIENCE （ 影响因子：2.217； 五年影响因子：2.376 ）

ISSN： 1594-4077

年卷期： 2007 年 6 卷

页码：

收录情况： SCI

摘要： This study investigated effects of beta-mercaptoethanol (beta-ME) and buffalo follicular fluid (buFF) on IVF and embryonic development of water buffalo oocytes derived from IVM. In Experiment 1, beta-ME (50 mu M and 100 mu M, treatments) was added in the media during IVM and IVC but not IVF. A highly significant difference was obtained in rate of blastocyst (9.26% Vs 26.72% or 27.04%, P<0.01), whereas no significant differences in rates of cleavage and hatched blastocyst between control group (0 mu M) and treatment groups (50 mu M and 100 mu M). Also, no significant differences (P>0.05) were observed in rates of cleavage, blastocyst and hatched blastocyst between two treatment groups, respectively. In Experiment 2, comparison with 10% FBS, significant and highly significant differences were recorded in rate of blastocyst in 5% FBS + 5% buFF (17.16% vs 27.64%, P<0.05)and 10% buFF (17.16% vs 32.98%, P<0.01), respectively. These results indicate that supplementation of beta-ME in IVM and IVC and replacement of FBS with buFF in IVM media improve remarkably the development of water buffalo embryos derived from IVM-IVF to the blastocyst stage.

分类号：

  • 相关文献

[1]Comparative Proteomic Analysis of Yak Follicular Fluid during Estrus. Guo, Xian,Pei, Jie,Ding, Xuezhi,Chu, Min,Bao, Pengjia,Wu, Xiaoyun,Liang, Chunnian,Yan, Ping.

[2]Proteomic profiling of follicle fluids after superstimulation in one-month-old lambs. Wu, Y.,Lin, J.,Han, B.,Wang, L.,Chen, Y.,Liu, M.,Huang, J.. 2018

[3]Proteome Profile and Quantitative Proteomic Analysis of Buffalo (Bubalusbubalis) Follicular Fluid during Follicle Development. Fu, Qiang,Huang, Yulin,Wang, Zhiqiang,Chen, Fumei,Huang, Delun,Lu, Yangqing,Zhang, Ming,Liang, Xianwei. 2016

[4]A Comparison of Milk Protein, Amino Acid and Fatty Acid Profiles of River Buffalo and Their F1 and F2 Hybrids with Swamp Buffalo in China. Ren, Da-xi,Chen, You-liang,Liu, Jian-xin,Zou, Cai-xia,Lin, Bo,Liang, Xian-wei. 2015

[5]OVUM PICK-UP-IN VITRO EMBRYO PRODUCTION - EMBRYO TRANSFER TECHNOLOGY IN THE APPLICATION OF WATER BUFFALO PRODUCTION. Qin, Guang-Sheng,Chen, Ming-Tang,Yang, Bing-Zhuang,Huang, Jia-xiang,Pang, Chun-ying,Zhang, Xiu-fang,Liang, Xian-wei,Tan, Zheng-zhun,Li, Hui,Jiang, He-sheng. 2012

[6]Rumen Bacterial Diversity of Water Buffalo (Bubalus bubalis) as Influenced by Concentrate Levels. Yang, Chengjian,Zou, Caixia,Liang, Xin,Wei, Shengju,Li, Shulu,Liang, Xianwei,Yang, Bingzhuang,Luo, Hua. 2013

[7]Vaccination with recombinant paramyosin in Montanide ISA206 protects against Schistosoma japonicum infection in water buffalo. Wu, Hannah Wei,Pond-tor, Sunthorn,Park, Sangshin,Kurtis, Jonathan D.,Fu, Zhi-Qiang,Lu, Ke,Hong, Yang,Li, Hao,Liu, Jin-Ming,Lin, Jiao-Jiao,Meng, Rui,Chu, Kai,Hou, Ming,Jiz, Mario,Meng, Rui,Chu, Kai.

[8]Molecular cloning and expression analysis of the STAT1 gene in the water buffalo (Bubalus bubalis). Deng, Tingxian,Pang, Chunying,Zhu, Peng,Yang, Bingzhuang,Liang, Xianwei,Deng, Tingxian,Pang, Chunying,Zhu, Peng,Yang, Bingzhuang,Liang, Xianwei,Liao, Biyun,Zhang, Ming.

[9]Rumen Bacterial Diversity of Murrah and Nili-Rivi Buffalo (Bubalus bubalis) Assessed by 454 GS FLX Pyrosequencing. Yang, Chengjian,Zou, Caixia,Liang, Xin,Wei, Shengju,Li, Shulu,Liang, Xianwei,Yang, Bingzhuang,Luo, Hua. 2013

[10]Transcriptomic responses of water buffalo liver to infection with the digenetic fluke Fasciola gigantica. Zhang, Fu-Kai,Zhang, Xiao-Xuan,He, Jun-Jun,Zheng, Wen-Bin,Ma, Jian-Gang,Guo, Ai-Jiang,Zhu, Xing-Quan,Elsheikha, Hany M.,Sheng, Zhao-An,Huang, Wei-Yi,Zhu, Xing-Quan. 2017

[11]Molecular Cloning, Identification, and Expression Patterns of Myostatin Gene in Water Buffalo (Bubalus Bubalis). Zhu, Peng,Li, Haiyang,Huang, Guiting,Cui, Jiayu,Zhang, Ruimen,Cui, Kuiqing,Yang, Sufang,Shi, Deshun,Zhu, Peng. 2018

[12]PRELIMINARY STUDY ON THE USE OF INHIBIN TO IMPROVE THE WATER BUFFALO SUPEROVULATION. Qin, Guang-Sheng,Abakar, Al-Kal,Pan, Bin,Chen, Bao-Jian,Mao, Xian-bao,Jiang, He-sheng,Wei, Ying-ming,Jiang, Qin-yang,Qin, Yong-Chang,Li, De-ru,Shi, Zhen-dan. 2012

[13]iTRAQ-Based Comparative Proteomic Analysis of Adult Schistosoma japonicum from Water Buffalo and Yellow Cattle. Zhai, Qi,Yu, Xingang,Li, Guoqing,Zhai, Qi,Fu, Zhiqiang,Hong, Yang,Yu, Xingang,Han, Qian,Lu, Ke,Li, Hao,Dou, Xuefeng,Zhu, Chuangang,Liu, Jinming,Lin, Jiaojiao. 2018

[14]Preliminary Study on Energy Metabolism and Energy Requirement of Early Lactation Water Buffalo. Zou, Caixia,Yang, Bingzhuang,Wei, Shengju,Liang, Xianwei,Li, Shulu,Qin, Guangsheng,Yang, Chenjian. 2013

[15]Effect of Cysteamine Hydrochloride on In Vitro Methane Emission in Water Buffalo. Zou, Caixia,Yang, Bingzhuang,Liang, Xianwei,Yang, Chengjian,Huang, Yali,Xia, Zhongsehng,Lu, Tianshui. 2013

[16]Nuclear transfer in leach (Paramisgurnus dabryanus Sauvage) by cell-to-cell electrofusion. Hongtuo, F,Chingjiang, W. 2001

[17]Effects of lipopolysaccharide on maturation of bovine oocyte in vitro and its possible mechanisms. Zhao, Shan-Jiang,Pang, Yun-Wei,Zhao, Xue-Ming,Du, Wei-Hua,Hao, Hai-Sheng,Zhu, Hua-Bin. 2017

[18]The CXCL12-CXCR4 signaling promotes oocyte maturation by regulating cumulus expansion in sheep. Zhang, Rui-Na,Pang, Bo,Guo, Song-Chang,Jia, Gong-Xue,Hu, Lin-Yong,Zhao, Xin-Quan,Yang, Qi-En,Xu, Shang-Rong,Wan, Peng-Cheng,Ji, Han-Zhong,Yang, Qi-En. 2018

[19]The Evaluation of Factors Affected on Juvenile in vitro Embryo Transfer in Xinjiang Fine Wool Lamb. Wu, Weiwei,Wang, Feng,Wu, Weiwei,Hanikezi,Tian, Kechuan,Xu, Xinming,Tian, Yuezhen. 2012

[20]Effect of mouse cumulus cells on the in vitro maturation and developmental potential of bovine denuded germinal vesicle oocytes. Zhao, Xue-Ming,Ren, Jing-Jing,Du, Wei-Hua,Hao, Hai-Sheng,Liu, Yan,Qin, Tong,Wang, Dong,Zhu, Hua-Bin.

作者其他论文 更多>>

微信小程序