Comparative analysis of the effects of different storage conditions on major royal jelly proteins

文献类型： 外文期刊

第一作者： Li, Jian-ke

作者： Li, Jian-ke;Wang, Ting;Peng, Wen-jun

作者机构：

关键词： royal jelly;apalbumins;storage temperature;2-DE

期刊名称：JOURNAL OF APICULTURAL RESEARCH （ 影响因子：2.584； 五年影响因子：2.52 ）

ISSN： 0021-8839

年卷期： 2007 年 46 卷 2 期

页码：

收录情况： SCI

摘要： An experiment was carried out to analyse changes in the protein components of royal jelly (RJ) under different storage conditions, based on two dimensional electrophoresis (2-DE). The proteins identified were compared to those proteins already identified in the proteome complement of the RJ. The results showed that the total detected protein spots were 75,45,63 and 69, with molecular weight in the range of 7.64-72.33 kDa, isoelectric point 4.95-8.70, in the 2-DE image of RJ protein components stored at -20 degrees C for 80 days, 4 degrees C for 80 days, room temperature for 30 days, room temperature for 80 days, respectively. The spot of major royal jelly protein, apalbumin 1, was saturated in all images in this experiment, indicating that temperature has no significant effects on it. The spots number and the quantity of apalbumin 2 and apalbumin 3 did not increase or decrease following the temperature trend, suggesting they are also sensitive to temperature. However, spots of apalbumin 4 and glucose oxidase were observed only in the image of -20 degrees C for 80 days, and spots of apalbumin 5 were detected in the images of -20 degrees C and 4 degrees C for 80 days, indicating they are the proteins most sensitive to storage temperature and thus may be potential freshness markers for RJ, and that the best way to maintain quality of RJ is under freezing conditions.

分类号：

  • 相关文献

[1]Proteomics analysis of major royal jelly protein changes under different storage conditions. Zhang, Zhao-hui,Pan, Ying-hong,Li, Jian-ke,Feng, Mao,Zhang, Lan.

[2]Effects of Modified Atmosphere Packaging and Storage Conditions on Freshness Maintenance of Cut Flowers of Oriental Lily 'Activa'. Wu, Xue Wei,Suh, Jeung Keun,Wang, Li Hua. 2013

[3]Quality changes and internal browning developments of summer pineapple fruit during storage at different temperatures. Zhang, Lubin,Hu, Huigang,Jia, Zhiwei,Gu, Hui,Gong, Deqiang,Xu, Hanbing,Wang, Junning,He, Quanguang. 2013

[4]Behavior of Salmonella spp. on fresh-cut tropical fruits. Ma, Chen,Li, Jianguo,Zhang, Qun.

[5]FTIR Assessment of the Secondary Structure of Proteins in Royal Jelly under Different Storage Conditions. Zhou Qun,Sun Su-qin,Wu Li-ming,Hu Fu-liang,Wu Li-ming,Zhou Xiao,Zhao Jing. 2009

[6]Proteome Comparison of Hypopharyngeal Gland Development between Italian and Royal Jelly-Producing Worker Honeybees (Apis mellifera L). Li Jianke,Feng Mao,Begna, Desalegn,Fang Yu,Zheng Aijuan. 2010

[7]Development and validation of a multiclass method for the quantification of veterinary drug residues in honey and royal jelly by liquid chromatography-tandem mass spectrometry. Jin, Yue,Zhang, Jinzhen,Zhao, Wen,Zhang, Wenwen,Wang, Lin,Zhou, Jinhui,Li, Yi,Jin, Yue,Zhang, Jinzhen,Zhao, Wen,Wang, Lin,Zhou, Jinhui,Li, Yi,Jin, Yue,Zhang, Jinzhen,Zhao, Wen,Wang, Lin,Zhou, Jinhui,Li, Yi. 2017

[8]Fast Determination of Royal Jelly Freshness by a Chromogenic Reaction. Zheng, Huo-Qing,Wei, Wen-Ting,Hu, Fu-Liang,Wu, Li-Ming,Dietemann, Vincent,Dietemann, Vincent. 2012

[9]Fast determination of 26 amino acids and their content changes in royal jelly during storage using ultra-performance liquid chromatography. Wu Liming,Zhou Jinhui,Xue Xiaofeng,Li Yi,Zhao Jing. 2009

[10]Determination of tryptophan in bee pollen and royal jelly by high-performance liquid chromatography with fluorescence detection. Zhang, Jin-zhen,Xue, Xiao-feng,Zhou, Jin-hui,Chen, Fang,Wu, Li-ming,Li, Yi,Zhao, Jing. 2009

[11]Geographical Influences on Content of 10-Hydroxy-trans-2-Decenoic Acid in Royal Jelly in China. Wei, Wen-Ting,Zheng, Huo-Qing,Hu, Fu-Liang,Hu, Yuan-Qiang,Cao, Lian-Fei,Hepburn, H. Randall. 2013

[12]In-Depth Phosphoproteomic Analysis of Royal Jelly Derived from Western and Eastern Honeybee Species. Han, Bin,Fang, Yu,Feng, Mao,Lu, Xiaoshan,Huo, Xinmei,Meng, Lifeng,Wu, Bin,Li, Jianke.

[13]Towards posttranslational modification proteome of royal jelly. Zhang, Lan,Fang, Yu,Li, Rongli,Feng, Mao,Han, Bin,Zhou, Tiane,Li, Jianke.

[14]Isolation and characterization of proteins and lipids from honeybee (Apis mellifera L.) queen larvae and royal jelly. Xu, Xiang,Gao, Yanxiang,Xu, Xiang.

[15]Brain Membrane Proteome and Phosphoproteome Reveal Molecular Basis Associating with Nursing and Foraging Behaviors of Honeybee Workers. Han, Bin,Fang, Yu,Feng, Mao,Hu, Han,Hao, Yue,Ma, Chuan,Huo, Xinmei,Meng, Lifeng,Zhang, Xufeng,Wu, Fan,Li, Jianke.

[16]Quantitative Neuropeptidome Analysis Reveals Neuropeptides Are Correlated with Social Behavior Regulation of the Honeybee Workers. Han, Bin,Fang, Yu,Feng, Mao,Hu, Han,Qi, Yuping,Huo, Xinmei,Meng, Lifeng,Wu, Bin,Li, Jianke.

[17]Hplc Determination Of Adenosine In Royal Jelly. Xue, Xiao Feng,Zhou, Jin Hui,Wu, Li Ming,Zhao, Jing,Wu, Li Ming,Fu, Liang Hu.

[18]In-Depth N-Glycosylation Reveals Species-Specific Modifications and Functions of the Royal Jelly Protein from Western (Apis mellifera) and Eastern Honeybees (Apis cerana). Feng, Mao,Fang, Yu,Han, Bin,Xu, Xiang,Fan, Pei,Hao, Yue,Qi, Yuping,Hu, Han,Huo, Xinmei,Meng, Lifeng,Wu, Bin,Li, Jianke,Fan, Pei.

[19]Hydrophilic interaction chromatography/tandem mass spectrometry for the determination of melamine in royal jelly and royal jelly lyophilized powder. Zhou, Jinhui,Zhao, Jing,Xue, Xiaofeng,Zhang, Jinzhen,Chen, Fang,Li, Yi,Wu, Liming,Li, Chenchen,Mi, Jianqiu.

[20]Royal jelly proteome comparison between A. mellifera ligustica and A. cerana cerana. Fang Yu,Feng Mao,Li Jianke.

作者其他论文 更多>>

微信小程序