Babesia gibsoni: An apical membrane antigen-1 homologue and its antibody response in the infected dogs

文献类型: 外文期刊

第一作者: Zhou, Jinlin

作者: Zhou, Jinlin;Yang, Jun;Zhang, Guohong;Nishikawa, Yoshifumi;Fujisaki, Kozo;Xuan, Xuenan

作者机构:

关键词: Babesia gibsoni;AMA-1;antibody response

期刊名称:EXPERIMENTAL PARASITOLOGY ( 影响因子:2.011; 五年影响因子:2.132 )

ISSN: 0014-4894

年卷期: 2006 年 114 卷 4 期

页码:

收录情况: SCI

摘要: A cDNA encoding the apical membrane antigen-1 (AMA-1) homologue was obtained by immunoscreening a cDNA expression library prepared from Babesia gibsoni merozoite mRNA. The complete nucleotide sequence of the gene was 2062 bp. Computer analysis suggested that the sequence contains an open reading frame of 1794 bp with a coding capacity of approximately 66 kDa. Based on the homology analysis, this putative protein was designated as B. gibsoni AMA-1 (BgAMA-1). The BgAMA-1 gene was expressed in the Escherichia coli BL21 strain and used as the antigen in Western blotting and the enzyme-linked immunosorbent assay (ELISA). The results indicated that BgAMA-1 was recognized as an immunodominant antigen by the host immune system and that it induced a strong antibody response only in chronic B. gibsoni infection in dogs; however, the antibody response could not be detected in the early infection stage (within 15 days). This phenomenon might be explained by the limited stimulation with the low-abundance protein in the early infection stage. This result shows that BgAMA-1 is a new member of the AMA-1 family and that its immune response is characteristic of canine B. gibsoni infection. (c) 2006 Elsevier Inc. All rights reserved.

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