Annotation and expression profile analysis of 2073 full-length cDNAs from stress-induced maize (Zea mays L.) seedlings
文献类型: 外文期刊
第一作者: Jia, Jinping
作者: Jia, Jinping;Fu, Junjie;Zheng, Jun;Zhou, Xin;Huai, Junling;Wang, Jianhua;Wang, Meng;Zhang, Ying;Chen, Xiaoping;Zhang, Jinpeng;Zhao, Jinfeng;Su, Zhen;Lv, Yuping;Wang, Guoying
作者机构:
关键词: maize;full-length cDNA;osmotic stress;gene expression;macroarray
期刊名称:PLANT JOURNAL ( 影响因子:6.417; 五年影响因子:7.627 )
ISSN: 0960-7412
年卷期: 2006 年 48 卷 5 期
页码:
收录情况: SCI
摘要: Full-length cDNAs are very important for genome annotation and functional analysis of genes. The number of full-length cDNAs from maize (Zea mays L.) remains limited. Here we report the construction of a full-length enriched cDNA library from osmotically stressed maize seedlings by using the modified CAP trapper method. From this library, 2073 full-length cDNAs (accession numbers DQ244142-DQ246214) were collected and further analyzed by sequencing from both the 5'- and 3'-ends. A total of 1728 (83.4%) sequences did not match known maize mRNA and full-length cDNA sequences in the GenBank database and represent new full-length genes. After alignment of the 2073 full-length cDNAs with 448 maize BAC sequences, it was found that 84 full-length cDNAs could be mapped to the BACs. Of these, 43 genes (51.2%) have been correctly annotated from the BAC clones, 37 genes (44.0%) have been annotated with a different exon-intron structure from our cDNA, and four genes (4.76%) had no annotations in the TIGR database. Expression analysis of 2073 full-length maize cDNAs using a cDNA macroarray led to the identification of 79 genes upregulated by stress treatments and 329 downregulated genes. Of the 79 stress-inducible genes, 30 genes contain ABRE, DRE, MYB, MYC core sequences or other abiotic-responsive cis-acting elements in their promoters. These results suggest that these cis-acting elements and the corresponding transcription factors take part in plant responses to osmotic stress either cooperatively or independently. Additionally, the data suggest that an ethylene signaling pathway may be involved in the maize response to drought stress.
分类号:
- 相关文献
作者其他论文 更多>>
-
Dynamic changes of quality characteristics during fermentation of orange-fleshed sweet potato alcoholic beverage
作者:Wu, Huimin;Fu, Qinquan;Tang, Tiantian;Liu, Yinqi;Ma, Xinyi;Zhao, Min;Zhou, Xin;Wang, Bin;Shi, Xuewei;Yang, Yiling;Wang, Zhangying;Yang, Yiling;Wang, Zhangying;Wang, Bin;Shi, Xuewei;Wang, Bin;Shi, Xuewei;Wang, Bin;Shi, Xuewei
关键词:Orange-fleshed sweet potato (OFSP); Alcoholic beverage; Antioxidant properties; Physicochemical indicators; Volatile compounds
-
Bioenhanced remediation of dibutyl phthalate contaminated black soil by immobilized biochar microbiota
作者:Tao, Yue;Wang, Yao;Cui, Yunhe;Sun, Rui;Zhang, Bo;Qu, Jianhua;Zhang, Ying;Cai, Hongguang
关键词:Biochar; DBP; Microbial remediation; Black soil; Soil nutrient
-
Simultaneous profiling of chromatin-associated RNA at targeted DNA loci and RNA-RNA Interactions through TaDRIM-seq
作者:Ding, Cheng;Chen, Guoting;Luan, Shiping;Gao, Runxin;Fan, Yudong;Zhang, Ying;Wang, Xiaoting;Li, Guoliang;Foda, Mohamed F.;Yan, Jiapei;Li, Xingwang;Chen, Guoting;Li, Guoliang;Chen, Guoting;Li, Guoliang;Foda, Mohamed F.;Li, Xingwang;Li, Xingwang
关键词:
-
Chemotaxis of rhizosphere Pseudomonas sp. induced by foliar spraying of lanthanum reduces cadmium uptake by pakchoi
作者:Wang, Meng;Yu, Lei;Wang, Jing;Qin, Luyao;Sun, Xiaoyi;Liu, Jiaxiao;Han, Yun;Chen, Shibao
关键词:Cadmium toxicity; Plant growth; Root exudates; Pseudomonas; Chemotaxis
-
Enhanced removal of tetracycline from water using MgO-modified g-C3N4 composite: Synthesis optimization and mechanism investigation
作者:Yu, Hui;Gao, Longfei;Zhang, Xinyuan;Zhang, Shuang;Chi, Wenshi;Zhang, Long;Li, Jianzhuo;Tian, Yushi;Zhang, Ying;Cai, Hongguang
关键词:Heterojunction; Photocatalysis; Tetracycline degradation; Wastewater treatment
-
Biodegradable film drip fertigation is more conducive to reducing the diversity and abundance of antibiotic resistance genes than plastic film drip fertigation
作者:Li, Yingjie;Yuan, Yuhan;Li, Cuilan;Zhang, Jinjing;Liu, Hang;Wang, Meng;Li, Qian;Zhang, Lei;Sun, Bo;Qin, Yubo
关键词:Antibiotic resistance genes; Biodegradable film drip fertigation; Bacterial diversity; HT-PCR
-
Pan-genome bridges wheat structural variations with habitat and breeding
作者:Jiao, Chengzhi;Hao, Chenyang;Xie, Yuxin;Zhao, Li;Li, Tian;Fu, Junjie;Hou, Jian;Liu, Hongxia;Liu, Xu;Jia, Jizeng;Mao, Long;Zhang, Xueyong;Jiao, Chengzhi;Wang, Xiue;Xie, Xiaoming;Wang, Zihao;Zhang, Yuqi;Guo, Weilong;Chen, Liyang;Garg, Vanika;Chitikineni, Annapurna;Appels, Rudi;Varshney, Rajeev K.;Dwivedi, Girish;Dwivedi, Girish;Appels, Rudi
关键词: