Establishment of optimal conditions for long-term culture of erythrocytic stages of Theileria uilenbergi

文献类型： 外文期刊

第一作者： Miranda, Joana P. G.

作者： Miranda, Joana P. G.;Nascimento, Elisabete M.;Cruz, Helder J.;Yin, Hong;Zweygarth, Erich;Oliva, Abel G.

作者机构：

期刊名称：AMERICAN JOURNAL OF VETERINARY RESEARCH （ 影响因子：1.156； 五年影响因子：1.303 ）

ISSN： 0002-9645

年卷期： 2006 年 67 卷 11 期

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收录情况： SCI

摘要： Objective-To establish optimal conditions for long-term culture of the erythrocytic stage of Theileria uilenbergi. Sample Population-Red blood cells from 3 splenectomized sheep experimentally infected with a blood stabilate of T uilenbergi. Procedures-Cultures of T uilenbergi were initiated by use of blood from experimentally infected sheep collected when parasites were detected in Giemsa-stained thin blood smears. Different culture conditions were tested to optimize in vitro growth of the organisms. Subcultures were performed at a ratio of 1:2, 1:4, and 1:8 when the percentage of parasitized erythrocytes (PPE) was at least 1% or when the initial PPE was doubled. Results-The optimal culture medium was HL-1 medium (a complete chemically defined medium) supplemented with 20% sheep serum and 0.75% chemically defined lipids. Optimal culture conditions included incubation in a humidified 2% O(2), 5% CO(2), and 93% N(2) atmosphere at 37 degrees C. Cultures of the merozoite stage of the parasite were continuously propagated in vitro for > 1 year. The PPE reached values of up to 3%. Conclusions and Clinical Relevance-Optimization of culture conditions to reach a high PPE seems worthwhile. The continuous propagation of T uilenbergi in culture allows the production of parasite material without infecting animals and provides a continuous laboratory source of parasites for further studies.

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