Mannose selection system used for cucumber transformation

文献类型: 外文期刊

第一作者: He, Zhengquan

作者: He, Zhengquan;Duan, ZhenZhen;Liang, Wei;Chen, Faju;Yao, Wei;Liang, Hongwei;Yue, Chaoyin;Sun, Zongxiu;Chen, Fan;Dai, Jianwu

作者机构:

关键词: Agrobacterium-mediated transformation;Cucumis sativus L;phosphomannose-isomerase;positive selection

期刊名称:PLANT CELL REPORTS ( 影响因子:4.57; 五年影响因子:4.463 )

ISSN: 0721-7714

年卷期: 2006 年 25 卷 9 期

页码:

收录情况: SCI

摘要: The selectable marker system, which utilizes the pmi gene encoding for phosphomannose-isomerase that converts mannose-6-phosphate to fructose-6-phosphate, was adapted for Agrobacterium-mediated transformation of cucumber (Cucumis sativus L.). Only transformed cells were capable of utilizing mannose as a carbon source. The highest transformation frequency of 23% was obtained with 10 g/l mannose and 10 g/l sucrose in the medium. Molecular, genetic analysis, and PMI activity assay showed that the regenerated shoots contained the pmi gene and the gene was transmitted to the progeny in a Mendelian fashion. The results indicated that the mannose selection system, which is devoid of the disadvantages of antibiotic or herbicide selection, could be used for cucumber Agrobacterium-mediated transformation.

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