文献类型: 外文期刊
第一作者: He, Zhengquan
作者: He, Zhengquan;Duan, ZhenZhen;Liang, Wei;Chen, Faju;Yao, Wei;Liang, Hongwei;Yue, Chaoyin;Sun, Zongxiu;Chen, Fan;Dai, Jianwu
作者机构:
关键词: Agrobacterium-mediated transformation;Cucumis sativus L;phosphomannose-isomerase;positive selection
期刊名称:PLANT CELL REPORTS ( 影响因子:4.57; 五年影响因子:4.463 )
ISSN: 0721-7714
年卷期: 2006 年 25 卷 9 期
页码:
收录情况: SCI
摘要: The selectable marker system, which utilizes the pmi gene encoding for phosphomannose-isomerase that converts mannose-6-phosphate to fructose-6-phosphate, was adapted for Agrobacterium-mediated transformation of cucumber (Cucumis sativus L.). Only transformed cells were capable of utilizing mannose as a carbon source. The highest transformation frequency of 23% was obtained with 10 g/l mannose and 10 g/l sucrose in the medium. Molecular, genetic analysis, and PMI activity assay showed that the regenerated shoots contained the pmi gene and the gene was transmitted to the progeny in a Mendelian fashion. The results indicated that the mannose selection system, which is devoid of the disadvantages of antibiotic or herbicide selection, could be used for cucumber Agrobacterium-mediated transformation.
分类号:
- 相关文献
作者其他论文 更多>>
-
One stone, two birds: Preparation of nutritionally fortified and easy-to-swallow 3D printed pea protein gel based on high internal phase emulsions
作者:Liang, Wei;Xie, Junhong;Zhao, Qiannan;Jiang, Chenghong;Zhan, Xiaofeng;Chen, Mingrong;Rong, Jianhua;Hu, Yang;Zhang, Lu;Li, Chuan;Liang, Wei;Hu, Yang;Liang, Wei;Hu, Yang
关键词:High internal phase emulsion; Pea protein isolate; Rheological behavior; Texture; 3D printing; Dysphagia food
-
3-methyl-4-nitrophenol disturbs the maternal-to-zygotic transition of early embryos by damaging mitochondrial function and histone modification
作者:Chen, Fan;Luo, An-Feng;Pan, Kai-Xin;Gu, Hao;Zhou, Chang-Fan;Zeng, Wei;Liu, Song;Molenaar, Adrian;Ren, Hong-Yan;Bi, Yan-Zhen;Bi, Yan-Zhen;Huo, Li-Jun;Molenaar, Adrian
关键词:PNMC; Early embryonic development; Maternal-to-zygotic transition; Zygotic genome activation; Mitochondria; Histone modification
-
Overexpression of miR-192 Inhibits In Vitro Porcine Embryo Development by Inducing Oxidative Stress Damage and Impairing Mitochondrial Function
作者:He, Fan;Li, Mingguo;Qi, Mengfan;Fu, Binbin;Zhang, Huapeng;Li, Qingchun;Huang, Tao;Li, Mingguo;Chen, Fan;Bi, Yanzhen;Zhou, Rong;Huang, Tao
关键词:pig; miR-192; early embryo development
-
Winter green manure cultivation benefits soil quality and ecosystem multifunctionality under upland paddy rotations in tropics
作者:Liu, Qi;Yao, Wei;Zeng, Zhaohai;Zang, Huadong;Liu, Qi;Yao, Wei;Qi, Zhiqiang;Liu, Qi;Yao, Wei;Yang, Yadong;Zeng, Zhaohai;Zang, Huadong;Zhou, Jie;Peixoto, Leanne;Mganga, Kevin Z.;Qi, Zhiqiang
关键词:Green manure; Winter fallow; Ecosystem multifunctionality; Cropping systems; Soil quality
-
Long-term organic farming improves the red soil quality and microbial diversity in subtropics
作者:Wen, Yongkang;Yao, Wei;Yu, Taobing;Cheng, Lang;Lin, Fangding;Yang, Yadong;Zeng, Zhaohai;Zang, Huadong;Wen, Yongkang;Yang, Yadong;Zeng, Zhaohai;Zang, Huadong;Zhang, Qing;Yang, Jida;Zhu, Hongye;Gunina, Anna;Gunina, Anna;Mganga, Kevin Z.
关键词:Organic farming; Organic duration; Conventional farming; Soil quality; Co-occurrence network
-
Increased seasonal fallow intensity enhanced the soil fertility of latosol in a tropical multi-cropping region
作者:Yao, Wei;Liu, Qi;Wen, Yongkang;Tian, Butao;Yang, Yadong;Zeng, Zhaohai;Zang, Huadong;Yao, Wei;Liu, Qi;Wen, Yongkang;Tian, Butao;Qi, Zhiqiang;Mganga, Kevin Z.;Zeng, Zhaohai;Zang, Huadong;Qi, Zhiqiang;Jones, Davey L.
关键词:Green manure; Vegetable field; Soil fertility; Enzymatic stoichiometry; Legume; Diversified cropping
-
Analysis of the Tolerance of 22 Malus baccata Accessions to Alkali Stress
作者:Zhang, Lu;Liu, Xuan;Dong, Lijuan;Liang, Wei;Ma, Fengwang;Li, Cuiying;Yu, Wenquan;Li, Zhengnan
关键词:alkali tolerance; ARC; Malus baccata; alkali stress