Association between melatonin receptor 1A gene and expression of reproductive seasonality in sheep

文献类型： 外文期刊

第一作者： Chu, MX

作者： Chu, MX;Cheng, DX;Liu, WZ;Fang, L;Ye, SC

作者机构：

关键词： sheep;reproductive seasonality;melatonin receptor 1A gene;PCR-RFLP

期刊名称：ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES （ 影响因子：2.509； 五年影响因子：2.604 ）

ISSN： 1011-2367

年卷期： 2006 年 19 卷 8 期

页码：

收录情况： SCI

摘要： To determine whether a link exists between reproductive seasonality and the structure of the melatonin receptor 1A (MTNRIA) gene, the latter was studied in nonseasonal estrous breeds (Small Tail Han and Hu ewes) and seasonal estrous breeds (Dorset, Suffolk and German Mutton Merino ewes). A large fragment of the exon 2 of the MTNRIA gene was amplified and a uniform fragment of 824 by was obtained in 239 ewes of five breeds. The 824 by PCR product was digested with restriction endonucleases Mnl I and Rsa I, and checked for the presence of restriction sites. The presence (allele M) or absence (allele m) of an Mnl I site at base position 605 led to three genotypes MM (236 bp/236 bp), Mm (236 by/303 bp) and nun (303 by/303 bp) in five sheep breeds. The presence (allele R) or absence (allele r) of a Rsa I site at base position 604 led to three genotypes RR (267 bp/267 bp), Rr (267 by/290 bp) and rr (290 by/290 bp) in five sheep breeds. Frequencies of MM and RR genotypes were obviously higher, and frequencies of mm and rr genotypes were obviously lower in nonseasonal estrous sheep breeds than in seasonal estrous sheep breeds. Sequencing revealed four mutations (G453T, G612A, G706A, C891T) in mm genotype compared to MM genotype and one mutation (C606T) in rr genotype compared to RR genotype. For polymorphic Mnl I and Rsa I cleavage sites, the differences of genotype distributions were very highly significant (p < 0.01) between Small Tail Han ewes and seasonal estrous sheep breeds. In each group, no significant difference (p > 0.05) was detected. These results preliminarily showed an association between MM, RR genotypes and nonseasonal estrus in ewes and an association between mm, rr genotypes and seasonal estrus in ewes.

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