Sequence characterization, expression profile, chromosomal localization and polymorphism of the porcine SMPX gene

文献类型： 外文期刊

第一作者： Guan, HP

作者： Guan, HP;Fan, B;Li, K;Zhu, MJ;Yerle, M;Liu, B

作者机构：

关键词： sequence analysis;physical mapping;polymorphism;promoter;porcine;SMPX

期刊名称：ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES （ 影响因子：2.509； 五年影响因子：2.604 ）

ISSN： 1011-2367

年卷期： 2006 年 19 卷 7 期

页码：

收录情况： SCI

摘要： The full-length eDNA of the Porcine SMPX gene was obtained by the rapid amplification of cDNA ends (RACE). The nucleotide sequences and the predicted protein sequences share high sequence identity with both human and mouse. The promoter of SMPX was sequenced and then analyzed to find the promoter binding sites. The reverse transcriptase-polymerase chain reaction (RTPCR) revealed that SMPX has a high level of expression in heart and skeletal muscle, a very low expression in lung and spleen and no expression in liver, kidney, fat and brain. Moreover, SMPX has a differential expression level in skeletal muscle, the expression in 65-day embryos being higher than other stages. The porcine SMPX was mapped to SSCXp24 by using a somatic cell hybrid panel (SCHP) and was found closely linked to SW1903 using the radiation hybrid panel IMpRH. An A/G single nucleotide polymorphism (PCRP-FLP) in the 3'-untranslated region (3'-UTR) was detected in eight breeds. The analysis of allele frequency distribution showed that introduced pig breeds (Duroc and Large White) have a higher frequency of allele A while in the Chinese indigenous pig breeds (Qingping pig, Lantang pig, YushanBlack pig, Large Black-White pig, Small Meishan) have a higher frequencies of allele G. The association analysis using an experimental population (188 pigs), which included two cross-bred groups and three pure-blood groups, suggested that the SNP genotype was associated with intramuscular fat content.

分类号：

  • 相关文献

[1]Molecular characterization and expression pattern of the porcine STARS, a striated muscle-specific expressed gene. Peng, Y. B.,Guan, H. P.,Fan, B.,Zhao, S. H.,Xu, X. W.,Li, K.,Zhu, M. J.,Liu, B.,Li, K.,Yerle, M.. 2008

[2]Promoter Characterization and Expression Pattern Analysis of Porcine TCAP Gene. Qiao, Mu,Wu, Huayu,Huang, Jingshu,Peng, Xianwen,Liu, Guisheng,Feng, Zheng,Mei, Shuqi. 2012

[3]Myeloid zinc finger 1 (MZF1) is the most important transcriptional factor for porcine follistatin promoter. Sun Ya-meng,Yang Xiu-qin,Liu Di,Wang Liang,Zhang Dong-jie,Liu Di. 2015

[4]Partial molecular characterization, expression pattern, polymorphism and association analysis of porcine SKP2 gene. Li, Y.,Zhao, S. H.,Peng, K. M.,Li, Y.,Yang, S. L.,Tang, Z. L.,Cui, W. T.,Mu, Y. L.,Chu, M. X.,Li, K.,Li, Y.,Wu, Z. F..

[5]Expression and SNP association analysis of porcine FBXL4 gene. Li, Y.,Yang, S. L.,Tang, Z. L.,Cui, W. T.,Mu, Y. L.,Chu, M. X.,Li, K.,Li, Y.,Li, Y.,Zhao, S. H.,Peng, K. M.,Wu, Z. F..

[6]Molecular cloning and expression pattern of the porcine 5-aminolevulinate synthase 1 (ALAS1) gene and its association with reproductive traits. Liu, L. Q.,Li, F. E.,Deng, C. Y.,Li, F. E.,Deng, C. Y.. 2016

[7]Expression characterization, polymorphism and chromosomal location of the porcine calsarcin-3 gene. Wang, Heng,Yang, Shulin,Tang, Zhonglin,Mu, Yulian,Cui, Wentao,Li, Kui. 2007

[8]Polymorphism detection of porcine PSMC3, PSMC6 and PSMD3 genes and their association with partial growth, carcass traits, meat quality and immune traits. Wang, YF,Yang, J,Yu, M,Liu, B,Fan, B,Zhu, MJ,Xiong, IA,Li, K. 2005

[9]Characterization and functional significance of polymorphisms in porcine Toll-like receptor (TLR) 5 gene. Li, Hai-tao,Yang, Xiu-qin,Wang, Liang,Liu, Di. 2012

[10]Functional characterization of genetic variants in the porcine TLR3 gene. Wang, L.,Chen, Y. C.,Li, H. T.,Yang, X. Q.,Wang, L.,Zhang, D. J.,Liu, D.. 2014

[11]Association between PCR-SSCP of bone morphogenetic protein 15 gene and prolificacy in Jining Grey goats. Chu, M. X.,Jiao, C. L.,He, Y. Q.,Wang, J. Y.,Liu, Z. H.,Chen, G. H..

[12]A new method for SNP discovery. Xu, Jian-Yong,Xu, Gen-Bo,Chen, Song-Lin,Xu, Jian-Yong,Xu, Gen-Bo.

[13]Subcellular localization, expression patterns, SNPs and association analyses of the porcine HUMMLC2B gene. Wang, Huan L.,Wang, Heng,Zhu, Zheng M.,Wang, Chen F.,Zhu, Meng J.,Mo, De L.,Yang, Shu L.,Li, Kui.

[14]Multiple structural aberrations and physical mapping of rye chromosome 2R introgressed into wheat. Lifang Zhuang,Peng Liu,Zhenqian Liu,Tingting Chen,Nan Wu,Ling Sun,Zengjun Qi.

[15]Delimitation of the PSH1(t) gene for rice purple leaf sheath to a 23.5 kb DNA fragment. Wang, Wen-Ying,Ding, Han-Feng,Li, Run-Fang,Liu, Xu,Zhang, Yu,Yao, Fang-Yin,Li, Guang-Xian,Jiang, Ming-Song.

[16]Major haplotype divergence including multiple germin-like protein genes, at the wheat Sr2 adult plant stem rust resistance locus. Mago, Rohit,Tabe, Linda,Upadhyaya, Narayana,Ellis, Jeffrey G.,Spielmeyer, Wolfgang,Vautrin, Sonia,Berges, Helene,Simkova, Hana,Kubalakova, Marie,Dolezel, Jaroslav,Kong, Xiuying,Breen, James,Appels, Rudi. 2014

[17]Fine mapping of Pa-6 gene for purple apiculus in rice. Liu, Xu,Sun, Xu,Wang, Wenying,Ding, Hanfeng,Liu, Wei,Yao, Fangyin,Li, Guangxian,Jiang, Mingsong,Zhu, Changxiang.

[18]A genetic linkage map of Pleurotus tuoliensis integrated with physical mapping of the de novo sequenced genome and the mating type loci. Gao, Wei,Qu, Jibin,Zhang, Jinxia,Chen, Qiang,Zhang, Yan,Huang, Chenyang,Gao, Wei,Qu, Jibin,Zhang, Jinxia,Chen, Qiang,Zhang, Yan,Huang, Chenyang,Sonnenberg, Anton. 2018

[19]Physical mapping of puroindoline b-2 genes and molecular characterization of a novel variant in durum wheat (Triticum turgidum L.). Chen, F.,Xu, H. -X.,Zhang, F. -Y.,Dong, Z. -D.,Zhan, K. -H.,Cheng, X. -Y.,Cui, D. -Q.,Xia, X. -C.,He, Z. -H.,He, Z. -H.,Wang, D. -W.. 2011

[20]Molecular characteristics of the porcine TIMD4 gene and its association analysis. Yuan, Jing,Tang, Zhong-Lin,Yang, Shulin,Li, Kui,Cao, Ji-Yue.

作者其他论文 更多>>

微信小程序