Binding of three Cry1A toxins in resistant and susceptible strains of cotton bollworm (Helicoverpa armigera)

文献类型： 外文期刊

第一作者： Wang, GR

作者： Wang, GR;Liang, GM;Wu, KM;Bai, LY;Ren, XG;Guo, YY

作者机构：

关键词： Helicoverpa armigera;Cry1A toxins;binding site;binding model;resistance

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：3.963； 五年影响因子：4.454 ）

ISSN： 0048-3575

年卷期： 2006 年 85 卷 2 期

页码：

收录情况： SCI

摘要： Evolution of resistance by pests is the greatest threat to the continuous success of the Bacillus thuringiensis (Bt) toxins used in conventional sprays or in transgenic plants. The most common mechanism of insect resistance to Bt is reduced binding of toxins to target sites in the brush border membrane of the larval mid-gut. In this paper, binding experiments were performed with three I-125-Cry1A toxins and the brush border membrane vesicles from Cry1Ac resistant or susceptible strains of Helicoverpa armigera. The homologous competition test showed that there was no significant difference in Cry1Ac-binding affinity, but the concentration of Cry1Ac-binding sites dramatically decreased in the resistant strain (R-t decreased from 5.87 +/- 1.40 to 2.23 +/- 0.80). The heterologous competition test showed that there were three Cry1Ac-binding sites in the susceptible strain. Among them, site I bound with all three Cry1A toxins, site 2 bound with both Cry1Ab and Cry1Ac, and site 3 only bound with Cry1Ac. In the Cry1Ac resistant strain, the binding capability of site 1 with Cry1Ab decreased and site 2 did not bind with Cry1Ac. It is suggested that the absence of one binding site is responsible for H. armigera resistance to Cry1Ac. This result also showed that the resistance fitted the "mode 1" pattern of Bt resistance described previously. (c) 2005 Elsevier Inc. All rights reserved.

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