A B-lectin receptor kinase gene conferring rice blast resistance

文献类型: 外文期刊

第一作者: Chen, Xuewei

作者: Chen, Xuewei;Shang, Junjun;Chen, Dexi;Lei, Cailin;Zou, Yan;Zhai, Wenxue;Liu, Guozhen;Xu, Jichen;Ling, Zhongzhuan;Cao, Gang;Ma, Bingtian;Wang, Yuping;Zhao, Xianfeng;Li, Shigui;Zhu, Lihuang

作者机构:

关键词: B-lectin;serine-threonine kinase;resistance gene;map-based cloning;rice blast;rice (Oryza sativa L.)

期刊名称:PLANT JOURNAL ( 影响因子:6.417; 五年影响因子:7.627 )

ISSN: 0960-7412

年卷期: 2006 年 46 卷 5 期

页码:

收录情况: SCI

摘要: Rice blast, caused by the fungal pathogen Magnaporthe grisea, is one of the most devastating diseases in rice worldwide. The dominant resistance gene, Pi-d2 [previously named Pi-d(t)2], present in the rice variety Digu, confers gene-for-gene resistance to the Chinese blast strain, ZB15. Pi-d2 was previously mapped close to the centromere of chromosome 6. In this study, the Pi-d2 gene was isolated by a map-based cloning strategy. Pi-d2 encodes a receptor-like kinase protein with a predicted extracellular domain of a bulb-type mannose specific binding lectin (B-lectin) and an intracellular serine-threonine kinase domain. Pi-d2 is a single-copy gene that is constitutively expressed in the rice variety Digu. Transgenic plants carrying the Pi-d2 transgene confer race-specific resistance to the M. grisea strain, ZB15. The Pi-d2 protein is plasma membrane localized. A single amino acid difference at position 441 of Pi-d2 distinguishes resistant and susceptible alleles of rice blast resistance gene Pi-d2. Because of its novel extracellular domain, Pi-d2 represents a new class of plant resistance genes.

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