Preparation and evaluation of anti-SARS coronavirus IgY from yolks of immunized SPF chickens

文献类型： 外文期刊

第一作者： Fu, Chao-Yang

作者： Fu, Chao-Yang;Huang, He;Wang, Xiao-Mei;Liu, Yong-Gang;Wang, Zhi-Guo;Cui, Shang-Jin;Gao, Hong-Lei;Li, Zan;Li, Jing-Peng;Kong, Xian-Gang

作者机构：

关键词： IgY;severe acute respiratory syndrome virus;SARS;neutralization activity

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN： 0166-0934

年卷期： 2006 年 133 卷 1 期

页码：

收录情况： SCI

摘要： Severe acute respiratory syndrome (SARS) is a recently discovered viral disease. characterized by fever. cough, acute fibrinous pneumonia and high infectivity. Specific pathogen-free (SPF) chickens were immunized with inactivated SARS coronavirus and their eggs were harvested at regular intervals. Yolk immunoglobulin (IgY) was extracted using the water dilution method, followed by further purification on a Sephadex G-75 column. SDS-polyacrylamide gel electrophoresis (SDS-PAGE), Western blot and neutralization test results showed that the IgY obtained was of a high purity and had a strong reactive activity with a neutralization titer of 1:640. Lyophilization and stability tests showed that lyophilized anti-SARS coronavirus IgY had promising physical properties, with no significant reduction in reactive activity and good thermal stability. All these data suggest that the anti-SARS coronavirus IgY could be a new useful biological product for specific antiviral therapy against SARS. (c) 2005 Elsevier B.V. All rights reserved.

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