Effect of the puroindoline locus and environment on Chinese fresh noodle texture

文献类型： 外文期刊

第一作者： Yang, EN

作者： Yang, EN;Zou, YC;Chen, DS;Sheppard, J;Martin, D;Huang, S;Mares, D;Sutherland, MW

作者机构：

关键词： Triticum aestivum;grain hardness;bulked segregant analysis;taste panel;Pinb noodle softness

期刊名称：AUSTRALIAN JOURNAL OF AGRICULTURAL RESEARCH （ 影响因子：1.328； 五年影响因子：1.781 ）

ISSN： 0004-9409

年卷期： 2006 年 57 卷 5 期

页码：

收录情况： SCI

摘要： Grain produced from doubled-haploid (DH) wheat lines, developed from a hard- and a soft-grained wheat cultivar, were bulked according to Pinb (puroindoline b) genotypes for an assessment of Chinese fresh noodle texture by a trained taste panel. Each DH line was designated as 'soft' or 'hard' grained, based on a PCR amplification of the wildtype, soft allele, or the mutant, hard allele. Theoretically, the soft and hard grain bulks represented respective Pinb alleles and an independent assortment of unlinked alleles from the parents, Sunco and Chuanyu 12. Grains from the parents and DH lines were grown at 2 locations in Queensland, Australia, and one in Sichuan, China. The grains were milled and processed for a taste panel evaluation in Chengdu, Sichuan. Results suggest the Pinb alleles had a significant effect on noodle softness and explained 30% of the variation; the 'soft' Pinb allele conferred a softer noodle texture. Location had a significant effect on noodle smoothness; wheat grain grown at Biloela, Queensland, produced a smoother noodle texture than grain grown in Sichuan. The effect of location confirms the importance of environment as a variable for this quality character. This investigation exemplifies the utility of Pinb markers for specifically altering Chinese Fresh Noodle texture.

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