A new puroindoline b mutation present in Chinese winter wheat cultivar Jingdong 11

文献类型： 外文期刊

第一作者： Chen, F

作者： Chen, F;He, ZH;Xia, XC;Lillemo, M;Morris, C

作者机构：

关键词： bread wheat;kernel hardness;puroindolines;Pinb mutation

期刊名称：JOURNAL OF CEREAL SCIENCE （ 影响因子：3.616； 五年影响因子：3.891 ）

ISSN： 0733-5210

年卷期： 2005 年 42 卷 2 期

页码：

收录情况： SCI

摘要： Kernel hardness is one of the most important characteristics in determining utilization and marketing of bread wheat. Genes coding for puroindoline a and b (PINA and PINB) were located at the Ha locus and designated as Pina-D1 and Pinb-D1, respectively. The coding sequence of the Pinb gene in a Chinese winter wheat cultivar Jingdong 11 (Triticum aestivum L.) was amplified with polymerase chain reaction (PCR), and the obtained 447-by fragment sequenced from two strands, and compared with the eight known Pinb alleles. The results showed that Jingdong 11 possessed a new Pinb allele not reported previously, and was designated as Pinb-D1q. It is characterized by a single base T to G substitution, which results in a tryptophan to leucine substitution (TGG to TTG) at position 44 and is most likely the cause of hard grain texture in Jingdong 11. The characterization of Pinb-D1 alleles would be helpful in manipulating grain hardness of bread wheat in breeding programs. (c) 2005 Elsevier Ltd. All rights reserved.

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