Homoeologous cloning of omega-secalin gene family in a wheat 1BL/1RS translocation

文献类型: 外文期刊

第一作者: Chai, JF

作者: Chai, JF;Liu, X;Jia, JZ

作者机构:

关键词: wheat;1BL/1RS translocation;omega-secalin;quality;homoeologous cloning

期刊名称:CELL RESEARCH ( 影响因子:25.617; 五年影响因子:25.924 )

ISSN: 1001-0602

年卷期: 2005 年 15 卷 8 期

页码:

收录情况: SCI

摘要: Wheat 1BL/1RS translocations are widely planted in China as well as in most of the wheat producing area in the world for their good qualities of disease resistance and high yield. 1BL/1RS translocations are however poor in bread making, partially caused by a family of small monomeric proteins, omega-secalins, which are encoded by genes on IRS. Based on published sequence of a rye omega-secalin gene we designed a pair of primers to cover the whole mature protein coding sequence. A major band could be amplified from 1BL/1RS translocations but not from euploid wheat. Using this primer set we conducted PCR amplification by using high fidelity Pfu polymerase on the genomic DNAs and cDNAs purified from a 1BL/1RS translocation Lankao 906. Sequencing analysis indicated that this gene family contains several members of 1150 bp, 1076 bp, 1075 bp, 1052 bp and 1004 bp genes, including two pseudogenes and three active genes. The gene transcripts were differentially expressed in developing seeds.

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