Effects of toll like receptor 4 overexpression on blood parameters, body composition, and gastrointestinal microbial diversity in sheep

文献类型： 外文期刊

第一作者： Song, Xu-Ting

作者： Song, Xu-Ting;Zhao, Duo-Wei;Lu, Qi;Lu, Jun;Yang, Xiu-Qin;Yao, Yu-Chang;Qi, Mei -Yu;Lu, Ming-Hai

作者机构：

关键词： genetically modified sheep; TLR4 gene; microbiomes; immune homeostasis; biosafety assessment

期刊名称：CZECH JOURNAL OF ANIMAL SCIENCE （ 影响因子：1.2； 五年影响因子：1.2 ）

ISSN： 1212-1819

年卷期： 2023 年 68 卷 5 期

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收录情况： SCI

摘要： Genetic modifications can improve the health status and disease resistance of farm animals. In the pre-vious study, genetically modified (GM) sheep overexpressing toll like receptor 4 (TLR4) were produced. The GM sheep had stronger ability to eliminate invasive microbes compared to the wild-type (WT) sheep. Physiological status and immune homeostasis, blood parameters, body composition, and gastrointestinal microbial diversity were evaluated in this study to elucidate the effects of TLR4 overexpression. In a set of 10 GM rams, the TLR4 mRNA and protein expression levels in the peripheral blood mononuclear cells (PBMCs), muscle, kidney, and spleen were higher than those of the WT (n = 10; P < 0.05). No significant differences in the parameters of red blood cells, white blood cells, platelets and in the composition of circulating T-lymphocyte subsets were observed between the GM and WT sheep (P > 0.05). Of the biochemical parameters, only total protein levels differed (were higher) in the GM sheep than in the WT sheep (P < 0.05) while no significant differences were observed for the other indices (P > 0.05). GM and WT sheep showed similar offal weights and muscle fatty acid and amino acid composi-tions (P > 0.05). Furthermore, TLR4 overexpression did not affect the community structure of the gastrointestinal tract microbiota. Eleven KEGG pathways associated with the TLR4 gene, physiology and biochemistry, growth and metabolism, fatty acids, and amino acids did not demonstrate significant differences between the GM and WT sheep (P > 0.05). In conclusion, TLR4 overexpression had no principal or adverse effects on the physiological development and health of sheep, except for the general mRNA and protein expression levels.

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