Establishment and identification of a Debao pony ear marginal tissue fibroblast cell line

文献类型： 外文期刊

第一作者： Zhou, XM

作者： Zhou, XM;Ma, YH;Guan, WJ;Zhao, DM

作者机构：

关键词： Debao pony;ear marginal tissue;fibroblast cell line;primary explant technique;collagenase technique

期刊名称：ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES （ 影响因子：2.509； 五年影响因子：2.604 ）

ISSN： 1011-2367

年卷期： 2004 年 17 卷 10 期

页码：

收录情况： SCI

摘要： The Debao pony ear marginal tissue fibroblast cell line (NDPEM 2/2) was successfully established using either primary explant technique or collagenase technique. The characterizations of the cell line were identified as following: the cells were adherent and of density limitation; population doubling time (PDT) of cells made with the two techniques were 35.9 h and 48 h, respectively; chromosome analysis showed that the frequency of cell chromosome number to be 2n=64 was 91.3%-92.8%. Confirmed by isoenzyme analysis, this cell line had no cross- contamination. Tests for microbial contamination from bacteria, fungi, virus or mycoplasma were negative. This newly established cell line meets all the standard quality controls of ATCC. It will provide a precious genetic resource for the conservation of the Debao pony breed, as well as effective experimental material for genetic studies on Debao ponies.

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