Targeted BSA mapping of Scmv1 and Scmv2 conferring resistance to SCMV using PstI/MseI compared with EcoRI/MseI AFLP markers

文献类型: 外文期刊

第一作者: Yuan, L

作者: Yuan, L;Dussle, CM;Muminovic, J;Melchinger, AE;Lubberstedt, T

作者机构:

关键词: Zea mays;AFLP;fine mapping;methylation;SCMV;targeted BSA

期刊名称:PLANT BREEDING ( 影响因子:1.832; 五年影响因子:1.956 )

ISSN: 0179-9541

年卷期: 2004 年 123 卷 5 期

页码:

收录情况: SCI

摘要: In a previous study, two chromosome regions (Scmv1 and Scmv2), conferring sugarcane mosaic virus (SCMV) resistance in maize, were enriched with EcoRI/MseI AFLP (Eco-AFLP) markers (methylation insensitive) by targeted bulked segregant analysis (tBSA). The objective of the present study was to further saturate these two regions with PstI/MseI AFLP (Pst-AFLP) markers (methylation sensitive) using the same tBSA approach, and to compare the genomic distribution of both Pst-AFLP and Eco-AFLP markers. Out of 470 PstI/MseI primer combinations screened, four Pst-AFLP markers were identified in the Scmv1 region (chromosome 6), and none in the Scmv2 region (chromosome 3). These Pst-AFLP markers were more closely linked to the Scmv1a gene than any of the Eco-AFLP markers, and could be useful for marker-assisted selection and even map-based cloning. In addition, Pst-AFLP and Eco-AFLP markers were dissimilarly distributed in both target regions. Pst-AFLP markers were equally distributed across both regions, while Eco-AFLPs were clustered in the Scmv2 region.

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