Targeted BSA mapping of Scmv1 and Scmv2 conferring resistance to SCMV using PstI/MseI compared with EcoRI/MseI AFLP markers
文献类型: 外文期刊
第一作者: Yuan, L
作者: Yuan, L;Dussle, CM;Muminovic, J;Melchinger, AE;Lubberstedt, T
作者机构:
关键词: Zea mays;AFLP;fine mapping;methylation;SCMV;targeted BSA
期刊名称:PLANT BREEDING ( 影响因子:1.832; 五年影响因子:1.956 )
ISSN: 0179-9541
年卷期: 2004 年 123 卷 5 期
页码:
收录情况: SCI
摘要: In a previous study, two chromosome regions (Scmv1 and Scmv2), conferring sugarcane mosaic virus (SCMV) resistance in maize, were enriched with EcoRI/MseI AFLP (Eco-AFLP) markers (methylation insensitive) by targeted bulked segregant analysis (tBSA). The objective of the present study was to further saturate these two regions with PstI/MseI AFLP (Pst-AFLP) markers (methylation sensitive) using the same tBSA approach, and to compare the genomic distribution of both Pst-AFLP and Eco-AFLP markers. Out of 470 PstI/MseI primer combinations screened, four Pst-AFLP markers were identified in the Scmv1 region (chromosome 6), and none in the Scmv2 region (chromosome 3). These Pst-AFLP markers were more closely linked to the Scmv1a gene than any of the Eco-AFLP markers, and could be useful for marker-assisted selection and even map-based cloning. In addition, Pst-AFLP and Eco-AFLP markers were dissimilarly distributed in both target regions. Pst-AFLP markers were equally distributed across both regions, while Eco-AFLPs were clustered in the Scmv2 region.
分类号:
- 相关文献
作者其他论文 更多>>
-
Genetic diversity among CIMMYT maize inbred lines investigated with SSR markers: II. Subtropical, tropical midaltitude, and highland maize inbred lines and their relationships with elite US and European maize
作者:Xia, XC;Reif, JC;Melchinger, AE;Frisch, M;Hoisington, DA;Beck, D;Pixley, K;Warburton, ML
关键词:
-
Genetic diversity among CIMMYT maize inbred lines investigated with SSR markers: I. Lowland tropical maize
作者:Xia, XC;Reif, JC;Hoisington, DA;Melchinger, AE;Frisch, M;Warburton, ML
关键词:
-
Genetic diversity determined within and among CIMMYT maize populations of tropical, subtropical, and temperate germplasm by SSR markers
作者:Reif, JC;Xia, XC;Melchinger, AE;Warburton, ML;Hoisington, DA;Beck, D;Bohn, M;Frisch, M
关键词:
-
QTL mapping of resistance to Sporisorium reiliana in maize
作者:Lubberstedt, T;Xia, XC;Tan, G;Liu, X;Melchinger, AE
关键词:maize;head smut;Sporisorium reiliana;QTL;RFLP;Ustilago maydis;Puccinia sorghi