Specific expression of a beta-tubulin gene (GhTub1) in developing cotton fibers

文献类型： 外文期刊

第一作者： Li, YL

作者： Li, YL;Sun, J;Li, CH;Zhu, YQ;Xia, GX

作者机构：

关键词： cotton;microtubules;beta-tubulin;fiber development

期刊名称：SCIENCE IN CHINA SERIES C-LIFE SCIENCES （ 影响因子：1.61； 五年影响因子：1.148 ）

ISSN： 1006-9305

年卷期： 2003 年 46 卷 3 期

页码：

收录情况： SCI

摘要： A cDNA library was constructed using poly (A)(+) RNA isolated from -1-15 DPA fibers of upland cotton (Gossypium hirsutum). The cDNA encoding a beta-tubulin isoform (designated as GhTub1) was identified through EST search. Northern blot analysis using 3'-UTR of the cDNA as a gene-specific probe was performed to investigate the expression levels of GhTub1 in various organs and in the developing fibers. The results showed that GhTub1 gene was specifically expressed in cotton fiber cells. During fiber development, GhTub1 transcripts accumulated highly at the stage of cell rapid elongation with the highest expression appearing at the time when fiber expansion reaches the peak rate. To probe the in vivo function of GhTub1, its cDNA was cloned in the yeast expression vector pREP1 and transformed into the fission yeast Schizosaccharomyces pombe. Overexpression of GhTub1 in yeast cells caused severe changes in the cell morphology. These results suggest that GhTub1 may play a role in the polar elongation,of cotton fibers. To our knowledge, this is the first report on the fiber-specific transcript accumulation of a cotton P-tubulin gene.

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