Establishment of a highly efficient transformation system for pepper (Capsicum annuum L.)

文献类型： 外文期刊

第一作者： Li, D

作者： Li, D;Zhao, K;Xie, B;Zhang, B;Luo, K

作者机构：

关键词： pepper;cotyledon;transformation;Agrobacterium

期刊名称：PLANT CELL REPORTS （ 影响因子：4.57； 五年影响因子：4.463 ）

ISSN： 0721-7714

年卷期： 2003 年 21 卷 8 期

页码：

收录情况： SCI

摘要： Application of modem genetic manipulation has been limited in pepper (Capsicum annuum L.) due to the lack of an efficient transformation system. Following the development of an efficient protocol for in vitro regeneration of pepper cotyledons, we investigated the key factors affecting transformation and established a highly efficient genetic transformation system using the pepper cotyledon as starting material. In this system, cotyledon explants are preconditioned for 2 days on kanamycin (km)-free DM1 medium [Murashige and Skoog (MS) salts/Gamborg B5 vitamins basal medium supplemented with 20 g/l sucrose, 5,000 mg/l DJ nutrients and a hormone combination of 1.0 mg/l indoleacetic acid (IAA) and 5.0 mg/l 6-benzyladenine (BA) solidified with 0.7% agar, pH 5.8], followed by co-cultivation with Agrobacterium tumefaciens on DM1 for 2 days and delay selection on DM1 with 500 nigh carbenicillin (carb) for 2 days. The explants are then placed on DM1 containing 10 mg/l AgNO3, 50 mg/l kin-sulfate and 500 mg/l carb. After 4-5 weeks, the explants with buds are transferred to EM1 medium (MS salts/Gamborg B5 vitamins basal medium supplemented with 20 g/l sucrose, 5,000 mg/l DJ nutrients, 10 mg/l AgNO3 and a hormone combination of 1.0 mg/l IAA, 3.0 mg/l BA and 2.0 mg/l gibberellic acid, solidified with 0.7% agar, pH 5.8) with 50 mg/l kanamycin and 500 mg/l carbenicillin for the elongation of buds. After 3-6 weeks, 1- to 2-cm-long elongated shoots are excised and planted on RM1 medium (MS basal medium supplemented with a hormone combination of 0.2 mg/l NAA and 0.1 mg/l IAA, solidified with 0.8% agar, pH 5.8) with 25 mg/l km and 200 mg/l carb for rooting. We tested four genotypes of pepper, and all presented a high differentiation efficiency (81.3% on average), elongation rate (61.5%) and rooting efficiency (89.5%). Polymerase chain reaction analysis results showed that 40.8% of the regenerated plantlets were transgenic plants.

分类号：

  • 相关文献

[1]Histological and Ultrastructural Observation Reveals Significant Cellular Differences between Agrobacterium Transformed Embryogenic and Non-embryogenic Calli of Cotton. Shang, Hai-Hong,Liu, Chuan-Liang,Zhang, Chao-Jun,Li, Feng-Lian,Hong, Wei-Dong,Li, Fu-Guang.

[2]A Comparative Proteomics Analysis of Soybean Cotyledon and Unifoliolate Leaves Under Heat (Chilling) Treatments. Jiang, Hong-wei,Xin, Da-wei,Shan, Cai-yun,Wang, Jin-hui,Chen, Qing-shan,Jiang, Hong-wei,Liu, Chun-yan,Hu, Guo-hua,Xin, Da-wei,Zhu, Rong-sheng,Hu, Zhen-bang,Chen, Qing-shan,Qiu, Hong-mei.

[3]Plant regeneration from cotyledon of Chieh-qua (Benincasa hispida Cogn. var. Chieh-qua How). He, Xiaoming,Xie, Dasen,Peng, Qingwu,Mu, Lixia. 2007

[4]Analysis of promoter activity reveals that GmFTL2 expression differs from that of the known Flowering Locus T genes in soybean. Liu, Limin,Mahi, Asia Adam Elzamzami,Wu, Xiaoxia,Chen, Qingshan,Zhang, Xiaomei,Chen, Fulu,Fu, Yong-Fu. 2017

[5]Production of transgenic apricot plants from hypocotyl segments of mature seeds. Petri, Cesar,Wang, Hong,Burgos, Lorenzo,Alburquerque, Nuria,Sanchez-Navarro, Jesus.

[6]Transcriptome Analysis of Maize Immature Embryos Reveals the Roles of Cysteine in Improving Agrobacterium Infection Efficiency. Liu, Yan,Zhang, Zhiqiang,Wang, Jianhua,Liu, Yan,Zhang, Zhiqiang,Fu, Junjie,Wang, Guoying,Liu, Yunjun. 2017

[7]Gene targeting using the Agrobacterium tumefaciens-mediated CRISPR-Cas system in rice. Xu, Rongfang,Li, Hao,Qin, Ruiying,Wang, Lu,Li, Li,Wei, Pengcheng,Yang, Jianbo,Li, Hao,Wei, Pengcheng,Xu, Rongfang,Li, Li. 2014

[8]Efficient Sugarcane Transformation via bar Gene Selection. Wang, W. Z.,Yang, B. P.,Feng, C. L.,Wang, J. G.,Xiong, G. R.,Zhao, T. T.,Zhang, S. Z.. 2017

[9]Arabidopsis AtPAP1 transcription factor induces anthocyanin production in transgenic Taraxacum brevicorniculatum. Qiu, Jian,Luo, Shiqiao,Xiao, Xianzhou,Liu, Shizhong,Sun, Shuquan,Zhang, Jichuan,Zhang, Liqun,Wang, Feng,Liu, Shizhong. 2014

[10]Bacterial artificial chromosome library construction of root-knot nematode resistant pepper genotype HDA149 and identification of clones linked to Me3 resistant locus. Guo Xiao,Yang Xiao-hui,Yang Yu,Ma Wei-qing,Li Guang-cun,Mao Zhen-chuan,Liu Feng,Xie Bing-yan,Li Guang-cun. 2017

[11]Cinnamaldehyde promotes root branching by regulating endogenous hydrogen sulfide. Xue, Yan-Feng,Zhang, Meng,Qi, Zhong-Qiang,Li, You-Qin,Shi, Zhiqi,Chen, Jian,Xue, Yan-Feng,Zhang, Meng,Qi, Zhong-Qiang,Li, You-Qin,Shi, Zhiqi,Chen, Jian.

[12]Preliminary study on the mutagenic effects of space flight on pepper seeds. Wang Dehui,Yu Ying,Guo Changhong,Guo Yahua. 2010

[13]Identification, Phylogeny, and Expression Analysis of Pto-like Genes in Pepper. Wan, Hongjian,Yuan, Wei,Ruan, Meiying,Ye, Qingjing,Wang, Rongqing,Li, Zhimiao,Zhou, Guozhi,Yao, Zhuping,Yang, Yuejian.

[14]Relationship of metabolism of reactive oxygen species with cytoplasmic male sterility in pepper (Capsicum annuum L.). Dai, Xiong-Ze,Zhang, Zhu-Qing,Zou, Xue-Xiao,Deng, Ming-Hua,Zhu, Hai-Shan,Zhou, Hui,Deng, Ming-Hua,Zou, Xue-Xiao,Wen, Jin-Fen,Huo, Jin-Long.

[15]A single-step reverse transcription loop-mediated isothermal amplification assay for rapid and accurate detection of Pepper vein yellows virus. Luo, Xiangwen,Zhang, Deyong,Liu, Yong,Luo, Xiangwen,Zhang, Deyong,Zhang, Songbai,Liu, Yong,Ouyang, Xian,Zhang, Zhanhong,Zhou, Xuguo,Li, Fan.

[16]Genetic analysis and identification of QTLs for resistance to cucumber mosaic virus in chili pepper (Capsicum annuum L.). Yao, Minghua,Ye, Zhibiao,Yao, Minghua,Li, Ning,Wang, Fei.

[17]MITIGATION OF WATERLOGGING-INDUCED DAMAGES TO PEPPER BY EXOGENOUS MeJA. Liu Zhou-Bin,Wei Ge,Yang Bo-Zhi,Zou Xue-Xia,Zou Chao-Hui,Zou Xue-Xia. 2017

[18]Impact of genotype, plant growth regulators and activated charcoal on embryogenesis induction in microspore culture of pepper (Capsicum annuum L.). Cheng, Yan,Ma, Rong-li,Jiao, Yan-sheng,Qiao, Ning. 2013

[19]Effect of refrigerator positions on quality of fresh-cut pepper. Ma, Y.. 2015

[20]Genome-Wide Identification and Expression Profile of Dof Transcription Factor Gene Family in Pepper (Capsicum annuum L.). Wu, Zhiming,Bang, Guansheng,Cheng, Jiaowen,Cui, Junjie,Hu, Kailin,Xu, Xiaowan,Luo, Xirong,Chen, Xiaocui,Tang, Xiangqun,Qin, Cheng,Qin, Cheng. 2016

作者其他论文 更多>>

微信小程序