Gene transfer strategy for kiwifruit to obtain pure transgenic plant through inducing adventitious roots in leaf explants with Agrobacterium tumefaciens

文献类型： 外文期刊

第一作者： Li, M

作者： Li, M;Huang, ZG;Han, LX;Zhao, GR;Li, YH

作者机构：

关键词： Actinidia deliciosa;Actinidia chinensis;transformation;chimera;gus gene

期刊名称：PROCEEDINGS OF THE FIFTH INTERNATIONAL SYMPOSIUM ON KIWIFRUIT

ISSN： 0567-7572

年卷期： 2003 年 610 期

页码：

收录情况： SCI

摘要： Owing to regeneration usually occurred via an intermediate callus phase and no adventitious buds directly regenerated from leaves in vitro in kiwifruit genetic transformation, kanamycin-resistant calli selected from leaf explants were usually chimeras with transformed and untransformed tissues. Reports have shown that adventitious roots were usually originated from a single cell in leaf explants. Based on these results, we attempted to obtain pure kiwifruit transgenic plants via inducing adventitious roots in leaf explants with Agrobacterium tumefaciens. The best re. generation of adventitious roots from leaf explants of kiwifruit was observed on MS medium with 2.0 mg/L IBA in continuous dark. Large adventitious roots were regenerated at the cut surface of each leaf explants in three weeks. In addition, rooting was observed in medium with 500 mg/L carbenicillin better than 500 mg/L cefotaxime. No adventitious roots were induced in medium with over 6 mg/L kanamycin. The leaf explants were co-cultivated with Agrobacterium tumefaciens strain A281 containing gus gene in medium with 2 mg/L IBA and 20 mg/L acetosyringone for 3 days in dark, then transferred to selective medium containing 2 mg/L IBA, 500 mg/L carbenicillin and 10 mg/L kanamycin, cultured in continuous dark. Kanamycin-resistant adventitious roots formed on leaf explants were excised and transferred to medium containing 2 mg/L zeatin, 0.1 mg/L IBA, 500 mg/L carbenicillin and 50 mg/L kanamycin to form calli and shoots, cultured under a 14h photoperiod. From 96 leaf explants we obtained 12 kanamycin-resistant calli. GUS histochemical staining assay indicated that these calli were pure transgenic tissues.

分类号：

  • 相关文献

[1]Toxicity of Actinidia chinensis extracts to Plutella xylostella. Chen, Kaoshan,Qi, Junshan,Li, Changsong,Li, Mei.

[2]Relationship between Omega as well as the length of 3 ' poly (dA) and efficiency of gene expression. Mao, LQ,Guo, SD. 1998

[3]Studies on transfer of Bt gene into Glycine max. Su, YH,Wang, HL,Yu, MM,Lu, DY,Guo, SD. 1999

[4]Histological and Ultrastructural Observation Reveals Significant Cellular Differences between Agrobacterium Transformed Embryogenic and Non-embryogenic Calli of Cotton. Shang, Hai-Hong,Liu, Chuan-Liang,Zhang, Chao-Jun,Li, Feng-Lian,Hong, Wei-Dong,Li, Fu-Guang.

[5]Generation of chimeric minipigs by aggregating 4-to 8-cell-stage blastomeres from somatic cell nuclear transfer with the tracing of enhanced green fluorescent protein. Ji, Huili,Long, Chuan,Feng, Chong,Shi, Ningning,Jiang, Yingdi,Zeng, Guomin,Li, Xirui,Wu, Jingjing,Pan, Dengke,Ji, Huili,Lu, Shengsheng,Long, Chuan,Lu, Lin. 2017

[6]Development and bioassay of transgenic Chinese cabbage expressing potato proteinase inhibitor II gene. Zhang, Junjie,Liu, Fan,Yao, Lei,Yin, Yue,Wang, Guixiang,Zhang, Junjie,Huang, Yubi,Luo, Chen. 2012

[7]Avoidance and Potential Remedy Solutions of Chimeras in Reconstructing the Phylogeny of Aphids Using the 16S rRNA Gene of Buchnera: A Case in Lachninae (Hemiptera). Chen, Rui,Wang, Zhe,Chen, Jing,Qiao, Ge-Xia,Chen, Rui,Wang, Zhe. 2015

[8]Generation of chimeric piglets by injection of embryonic germ cells from inbred Wuzhishan miniature pigs into blastocysts. Dong, Xiao,Mu, Yulian,Liu, Lixin,Chen, Hongping,Zhang, Li,Feng, Shutang,Dong, Xiao,Tsung, Hsiaochien,Mu, Yulian,Feng, Shutang,Wang, Hongjun. 2014

[9]Barriers for Deriving Transgene-Free Pig iPS Cells with Episomal Vectors. Du, Xuguang,Feng, Tao,Yu, Dawei,Zou, Huiying,Ma, Shuangyu,Hu, Xiaoxiang,Li, Ning,Wu, Sen,Wu, Yuanyuan,Feng, Chong,Pan, Dengke,Huang, Yongye,Ouyang, Hongsheng.

[10]Heritable variation and small RNAs in the progeny of chimeras of Brassica juncea and Brassica oleracea. Li, Junxing,Wang, Yan,Zhang, Langlang,Liu, Bin,Cao, Liwen,Qi, Zhenyu,Chen, Liping,Li, Junxing,Zhang, Langlang,Liu, Bin,Cao, Liwen,Chen, Liping,Wang, Yan,Qi, Zhenyu.

[11]Variant Amino Acid Residues Alter the Enzyme Activity of Peanut Type 2 Diacylglycerol Acyltransferases. Zheng, Ling,Wan, Shubo,Peng, Zhenying,Zheng, Ling,Bian, Fei,Chen, Gao,Shan, Lei,Li, Xinguo,Wan, Shubo,Peng, Zhenying,Shockey, Jay. 2017

[12]Transformation of Liquidambar formosana L. via Agrobacterium tumefaciens using a mannose selection system and recovery of salt tolerant lines. Qiao, Guirong,Zhou, Jing,Jiang, Jing,Sun, Yuehua,Pan, Luanyin,Song, Honggai,Jiang, Jingmin,Zhuo, Renying,Sun, Yuehua,Wang, Xiaojuan,Sun, Zongxiu,Sun, Yuehua. 2010

[13]Improvement of Agrobacterium-mediated transformation efficiency of maize (Zea mays L.) genotype Hi-II by Optimizing Infection and Regeneration Conditions. Xu, You,Ren, Wen,Liu, Ya,Zhao, Jiuran,Xu, You. 2016

[14]Progress in chloroplast genome analysis. Liu, Clarke Jihong,Xing Shao-Chen. 2008

[15]Protoplast transformation as a potential platform for exploring gene function in Verticillium dahliae. Rehman, Latifur,Su, Xiaofeng,Guo, Huiming,Qi, Xiliang,Cheng, Hongmei. 2016

[16]Production of Bioactive Recombinant Bovine Chymosin in Tobacco Plants. Wei, Zheng-Yi,Fan, Ming-Xia,Lin, Feng,Wei, Zheng-Yi,Zhang, Yu-Ying,Wang, Yun-Peng,Zhong, Xiao-Fang,Xing, Shao-Chen,Zhang, Yu-Ying,Xu, Nuo. 2016

[17]Regeneration of Chinese cabbage transgenic plants expressing antibacterial peptide gene and cowpea trypsin inhibitor gene. Zhao, Junliang,Liang, Aihua,Zhu, Zhen,Tang, Yixiong. 2006

[18]Transformation of sulfate and organic S in rice straw in flooded paddy soils and its availability to plants using sulfur-35 labeling. Zhou, W,Li, ST,He, P,Lin, B. 2006

[19]A new time-saving transformation system for Brassica napus. Kong, Fanming,Li, Juan,Tan, Xiaoli,Zhang, Lili,Zhang, Zhiyan,Ma, Xiaoke,Qi, Cunkou. 2009

[20]Transient beta-glucuronidase expression in asparagus protoplasts following electroporation. Chen, GY,Conner, AJ,Fautrier, AG,Field, RJ. 1999

作者其他论文 更多>>

微信小程序