The study on the interaction between band3 C-ternimus and glycophorin A and their immunologic cross-link relation

文献类型： 外文期刊

第一作者： Du, HQ

作者： Du, HQ;Fu, GH;Jiang, XS;Long, XY

作者机构：

关键词： glycophorin A;band3;baculovirus expression system;membrane protein

期刊名称：PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS （ 影响因子：0.351； 五年影响因子：0.272 ）

ISSN： 1000-3282

年卷期： 2002 年 29 卷 2 期

页码：

收录情况： SCI

摘要： The correlation of glycophorin A and band3 was confirmed by purification and identification of hydrophilic peptides of red blood cell transmembrane domain with high performance liquid chromatograghy (HPLC). Glycophorin A gene (410 bp) was amplified from K562 with RT-PCR and subcloned into the yeast BD hybrid vector pGBKT(7) and baculovirus transfer vector pFASTBac. Glycophorin A and band3 C-terminus were co-transformed into the yeast strain AH109 and the interaction was identified by nutritional selection and beta-glycotosidase activity detection. The GPA expression product in Sf9 cells was analyzed on Western blot with anti-GPA and anti-human band3 antibodies, and the result showed that they have immunologic cross-link reaction. All the results above confirmed the interaction between band3 and glycophorin A.

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